Kinetic and structural insights into enzymatic mechanism of succinic semialdehyde dehydrogenase from Cyanothece sp. ATCC51142.
Kinetic and structural insights into enzymatic mechanism of succinic semialdehyde dehydrogenase from Cyanothece sp. ATCC51142.
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对 Cyanothece sp 琥珀酸半醛脱氢酶酶促机制的动力学和结构见解。
DOI：
10.1371/journal.pone.0239372
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Xie C;Li ZM;Bai F;Hu Z;Zhang W;Li Z
As a ubiquitous enzyme, succinic semialdehyde dehydrogenase contributes significantly in many pathways including the tricarboxylic acid cycle and other metabolic processes such as detoxifying the accumulated succinic semialdehyde and surviving in nutrient-limiting conditions. Here the cce4228 gene encoding succinic semialdehyde dehydrogenase from Cyanothece sp. ATCC51142 was cloned and the homogenous recombinant cce4228 protein was obtained by Ni-NTA affinity chromatography. Biochemical characterization revealed that cce4228 protein displayed optimal activity at presence of metal ions in basic condition. Although the binding affinity of cce4228 protein with NAD+ was about 50-fold lower than that of cce4228 with NADP+, the catalytic efficiency of cce4228 protein towards succinic semialdehyde with saturated concentration of NADP+ is same as that with saturated concentration of NAD+ as its cofactors. Meanwhile, the catalytic activity of cce4228 was competitively inhibited by succinic semialdehyde substrate. Kinetic and structural analysis demonstrated that the conserved Cys262 and Glu228 residues were crucial for the catalytic activity of cce4228 protein and the Ser157 and Lys154 residues were determinants of cofactor preference.
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影响因子：
11
作者：
Falcon, Luisa I.;Magallon, Susana;Castillo, Amanda
通讯作者：
Castillo, Amanda
影响因子：
3.2
作者：
Fuhrer, Tobias;Chen, Lifeng;Vitkup, Dennis
通讯作者：
Vitkup, Dennis
DOI：
10.1073/pnas.0805418105
发表时间：
2008-09-30
影响因子：
11.1
作者：
Welsh, Eric A.;Liberton, Michelle;Pakrasi, Himadri B.
通讯作者：
Pakrasi, Himadri B.
影响因子：
3.9
作者：
de Carvalho LP;Ling Y;Shen C;Warren JD;Rhee KY
通讯作者：
Rhee KY
影响因子：
3.8
作者：
Jang EH;Park SA;Chi YM;Lee KS
通讯作者：
Lee KS