Serum miR-1290 and miR-1246 as Potential Diagnostic Biomarkers of Human Pancreatic Cancer
Serum miR-1290 and miR-1246 as Potential Diagnostic Biomarkers of Human Pancreatic Cancer
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血清 miR-1290 和 miR-1246 作为人类胰腺癌的潜在诊断生物标志物
DOI：
10.7150/jca.38048
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发表时间：
2020-01
影响因子：
3.9
通讯作者：
Xu Jian
中科院分区：
文献类型：
--
作者：
Wei Jia;Yang Lu;Wu Yi-ning;Xu Jian
背景：胰腺癌是一种高度恶性的肿瘤，其早期诊断缺乏有效的生物标志物。本研究旨在筛选和鉴定血清microRNAs(MiRNAs)作为PC诊断的非侵入性生物标志物。方法：通过对三个独立的GEO数据集的综合分析，筛选出两个上调的miRNAs。采用定量逆转录聚合酶链式反应检测120例PC患者、40例良性疾病患者和40例健康人血清中两种miRNAs的表达。分析血清miRNAs水平与临床特征的相关性。应用受试者工作特征曲线分析比较miRNAs与CA19-9的诊断价值。结果：通过对GEO数据集的分析，我们发现miR-1290和miR-1246在PC患者中上调。前列腺癌患者血清miR-1290和miR-1246表达水平均高于所有对照组，肿瘤切除后显著降低(均P<；0.001)。MiR-1290的曲线下面积(AUC)大于miR-1246和CA19-9(miR-1290：0.91；miR-1246：0.81；CA19-9：0.82)。单项或两项miRNAs与CA19-9联合检测对PC和所有对照组的鉴别诊断均优于CA19-9单独检测(miR-1290+CA19-9：0.96，miR-1246+CA19-9：0.93，miR-1290+miR-1246+CA19-9：0.97)。血清miR-1290和miR-1246的丰度分别与肿瘤分期和肿瘤大小有关，Logistic回归分析表明两者均为PC的独立危险因素。结论：血清miR-1290和miR-1246可能是诊断PC的良好生物标志物，CA19-9与miR-1290或miR-1246联合检测可提高PC的诊断准确率。
Background: Pancreatic cancer (PC) is a highly malignant tumor with no effective early diagnostic biomarkers. This study was performed to screen and identify serum microRNAs (miRNAs) as noninvasive biomarkers for PC diagnosis. Methods: Two upregulated miRNAs were selected by integrated analysis of three independent GEO datasets. Then, the expressions of two miRNAs in serum were determined by quantitative reverse-transcription PCR among 120 PC patients, 40 benign disease controls and 40 healthy controls. The correlation between serum miRNAs and clinical characteristics was analyzed. The diagnostic utility of miRNAs was compared to CA19-9 using receiver operating characteristic curve analysis. Results: We discovered miR-1290 and miR-1246 were upregulated in PC patients through GEO datasets analysis. Serum miR-1290 and miR-1246 expression levels were elevated in PC patients compared to all controls and dramatically decreased after tumor resection (all P<0.001). The area under the curve (AUC) for miR-1290 was larger than miR-1246 and CA19-9 (miR-1290: 0.91; miR-1246: 0.81; CA19-9: 0.82). The combined diagnosis of individual or both miRNAs with CA19-9 was more effective for discriminating PC from all controls than the single CA19-9 assay (miR-1290+CA19-9: 0.96, miR-1246+CA19-9: 0.93, miR-1290+miR-1246+CA19-9: 0.97). The abundance of serum miR-1290 and miR-1246 was associated with tumor stage and size respectively and logistic modeling proved that both of them were independent risk factors for PC. Conclusion: Serum miR-1290 and miR-1246 might be promising biomarkers for PC diagnosis and the combined detection of CA19-9, together with miR-1290 or miR-1246, could improve the diagnostic accuracy of PC.
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DOI：
10.1146/annurev.pathol.3.121806.154305
发表时间：
2021-08
期刊：
Annual review of pathology
影响因子：
--
作者：
A. Maitra;R. Hruban
通讯作者：
A. Maitra;R. Hruban
DOI：
10.1146/annurev.pathol.4.110807.092222
发表时间：
2009
期刊：
Annual review of pathology
影响因子：
--
作者：
Lee YS;Dutta A
通讯作者：
Dutta A
DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
MicroRNA-1246与CCNG2介导的化学耐药性和胰腺癌的干性相关的表达。
影响因子：
8.8
作者：
Hasegawa, S.;Eguchi, H.;Nagano, H.;Konno, M.;Tomimaru, Y.;Wada, H.;Hama, N.;Kawamoto, K.;Kobayashi, S.;Nishida, N.;Koseki, J.;Nishimura, T.;Gotoh, N.;Ohno, S.;Yabuta, N.;Nojima, H.;Mori, M.;Doki, Y.;Ishii, H.
通讯作者：
Ishii, H.
影响因子：
3.7
作者：
Ogata-Kawata H;Izumiya M;Kurioka D;Honma Y;Yamada Y;Furuta K;Gunji T;Ohta H;Okamoto H;Sonoda H;Watanabe M;Nakagama H;Yokota J;Kohno T;Tsuchiya N
通讯作者：
Tsuchiya N