Gene losses and partial deletion of small single-copy regions of the chloroplast genomes of two hemiparasitic Taxillus species.
Gene losses and partial deletion of small single-copy regions of the chloroplast genomes of two hemiparasitic Taxillus species.
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DOI：
10.1038/s41598-017-13401-4
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发表时间：
2017-10-12
影响因子：
4.6
通讯作者：
Yao H
中科院分区：
文献类型：
--
作者：
Li Y;Zhou JG;Chen XL;Cui YX;Xu ZC;Li YH;Song JY;Duan BZ;Yao H
已知寄生植物的叶绿体基因组中存在许多变异。本文测定了两种半寄生植物-中国寄生sutchuenensis，使用Illumina和PacBio测序技术。这些物种是桑寄生科的第一个被测序的成员。本文报道了T. chinensis和T. Sutchuenensis分别包含具有四分结构的121，363和122，562 bp长的环状分子。与普通烟草（Nicotianatabacum）和白芥（Occinisalba）叶绿体基因组相比，这两个物种的所有ndh基因以及3个核糖体蛋白基因、7个tRNA基因、4个ycf基因和infA基因均丢失。最大似然法和邻接系统树的结果支持桑寄生科和槲寄生科是单系分支的理论。本研究揭示了寄生生活方式对T. chinensis和T. sutchuenensis，并增强了我们对Illumina和PacBio之间组装结果差异的理解。
Numerous variations are known to occur in the chloroplast genomes of parasitic plants. We determined the complete chloroplast genome sequences of two hemiparasitic species, Taxillus chinensis and T. sutchuenensis, using Illumina and PacBio sequencing technologies. These species are the first members of the family Loranthaceae to be sequenced. The complete chloroplast genomes of T. chinensis and T. sutchuenensis comprise circular 121,363 and 122,562 bp-long molecules with quadripartite structures, respectively. Compared with the chloroplast genomes of Nicotiana tabacum and Osyris alba, all ndh genes as well as three ribosomal protein genes, seven tRNA genes, four ycf genes, and the infA gene of these two species have been lost. The results of the maximum likelihood and neighbor-joining phylogenetic trees strongly support the theory that Loranthaceae and Viscaceae are monophyletic clades. This research reveals the effect of a parasitic lifestyle on the chloroplast structure and genome content of T. chinensis and T. sutchuenensis, and enhances our understanding of the discrepancies in terms of assembly results between Illumina and PacBio.
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