Identification and characterization of two members of the FtsH gene family in maize (Zea mays L.)
Identification and characterization of two members of the FtsH gene family in maize (Zea mays L.)
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玉米 FtsH 基因家族两个成员的鉴定和表征 (Zea mays L.)
DOI：
10.1007/s11033-009-9691-3
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发表时间：
2010-02
影响因子：
2.8
通讯作者：
Zhang, Juren
中科院分区：
文献类型：
--
作者：
Yue, Guidong;Yang, Aifang;Hu, Xiaorui;He, Ying;Zhang, Juren
从玉米（Zea mays L.）通过抑制性消减杂交结合电子克隆方法。ZmFtsH 2A和ZmFtsH 2B蛋白均由677个氨基酸残基组成，与拟南芥FtsH 2蛋白酶具有高度的相似性。DNA凝胶印迹分析表明AtFtsH 2-like基因在玉米基因组中以双拷贝形式存在。克隆了ZmFtsH 2A和ZmFtsH 2B的基因组序列，其主要区别在于ZmFtsH 2B的第一内含子比ZmFtsH 2A长得多。RT-PCR分析表明，ZmFtsH 2A和ZmFtsH 2B基因在所有组织中均组成型表达，且ZmFtsH 2B基因的表达量高于ZmFtsH 2A基因。结果表明，ZmFtsH 2B基因在叶片中的转录水平在水分亏缺胁迫和阿坝处理下均显著上调，而ZmFtsH 2A基因在叶片和根系中均组成型表达。与野生型对照相比，过表达ZmFtsH 2A和ZmFtsH 2B的转基因烟草的耐旱性没有提高，这表明这两个基因可能不直接参与植物耐旱性，或者在这种条件下功能性FtsH杂合物的数量可能没有增加。本研究为玉米FtsH蛋白的进一步研究提供了基础信息。
Two full-length cDNAs, designated as ZmFtsH2A and ZmFtsH2B, were isolated from maize (Zea mays L.) by suppression subtractive hybridization coupled with in silico cloning approach. The predicted proteins of ZmFtsH2A and ZmFtsH2B both consisted of 677 amino acid residues and displayed high similarity to FtsH2 protease of Arabidopsis thaliana. DNA gel blotting analysis indicated that AtFtsH2-like genes exist as two copies in maize genome. The genomic sequences of ZmFtsH2A and ZmFtsH2B were cloned and the main difference was that the first intron of ZmFtsH2B was much longer than that of ZmFtsH2A. RT-PCR analysis revealed that both genes were constitutively expressed in all examined tissues and the expression level of ZmFtsH2B transcripts was higher than that of ZmFtsH2A. The responses of the two genes in maize seedlings to PEG, cold, high salt, and ABA treatments were compared, and the results showed that ZmFtsH2B transcription in leaves was markedly up-regulated by water deficit stress and ABA treatments while ZmFtsH2A constitutively expressed both in leaves and roots under all tested stressful conditions. Drought tolerance of transgenic tobaccos overexpressing ZmFtsH2A and ZmFtsH2B weren’t improved compared to wild-type controls, which indicated that two genes might not be directly involved in plant drought tolerance or the number of functional FtsH heterocomplex might not be increased in this condition. Our current study provides fundamental information for the further investigation of the maize FtsH proteins.
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影响因子：
7
作者：
A. F. Neuwald;L. Aravind;J. Spouge;E. Koonin
通讯作者：
A. F. Neuwald;L. Aravind;J. Spouge;E. Koonin
影响因子：
4.3
作者：
Komayama, Keisuke;Khatoon, Mahbuba;Yamamoto, Yasusi
通讯作者：
Yamamoto, Yasusi
DOI：
10.1111/j.1365-313x.2003.02014.x
发表时间：
2004-03
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Fei Yu;Sungsoon Park;S. Rodermel
通讯作者：
Fei Yu;Sungsoon Park;S. Rodermel
影响因子：
2.5
作者：
A. Sokolenko;E. Pojidaeva;V. Zinchenko;V. Panichkin;V. Glaser;R. Herrmann;S. Shestakov
通讯作者：
A. Sokolenko;E. Pojidaeva;V. Zinchenko;V. Panichkin;V. Glaser;R. Herrmann;S. Shestakov
影响因子：
3.2
作者：
SANTOS, D;DEALMEIDA, DF
通讯作者：
DEALMEIDA, DF