Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice.
Swine-origin pandemic H1N1 influenza virus-like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice.
复制标题
DOI：
10.1002/biot.200900267
复制
发表时间：
2010-01
影响因子：
4.7
通讯作者：
Grabherr, Reingard
中科院分区：
文献类型：
--
作者：
Krammer, Florian;Nakowitsch, Sabine;Messner, Paul;Palmberger, Dieter;Ferko, Boris;Grabherr, Reingard
最近甲型流感的爆发突出了迅速和充分供应大流行和大流行间疫苗的重要性。传统的流感疫苗制造方法无法满足这一需求。另外，已经建立了基于细胞培养的生产系统和基于病毒样颗粒（VLP）的技术。我们开发了由血凝素（A/California/04/2009）和基质蛋白组成的猪源性大流行性H1N1流感VLPs。利用杆状病毒表达系统在昆虫细胞中共表达血凝素和基质蛋白。从感染上清中收集VLPs，纯化后用于BALB/c小鼠腹腔免疫。免疫诱导高血清抗体滴度对抗A/California/04/2009以及血凝抑制抗体。此外，我们比较了两种不同昆虫细胞系Sf9和BTI-TN5B1-4 （High Five™）的VLP产量。综上所述，VLPs代表了一种对抗新型大流行性流感病毒的潜在战略。
Recent outbreaks of influenza A highlight the importance of rapid and sufficient supply for pandemic and inter-pandemic vaccines. Classical manufacturing methods for influenza vaccines fail to satisfy this demand. Alternatively, cell culture-based production systems and virus-like particle (VLP)-based technologies have been established. We developed swine-origin pandemic H1N1 influenza VLPs consisting of hemagglutinin (A/California/04/2009) and matrix protein. Hemagglutinin and matrix protein were co-expressed in insect cells by the baculovirus expression system. VLPs were harvested from infection supernatants, purified and used for intraperitoneal immunization of BALB/c mice. Immunization induced high serum antibody titers against A/California/04/2009 as well as hemagglutination inhibiting antibodies. Additionally, we compared VLP production in two different insect cell lines, Sf9 and BTI-TN5B1-4 (High Five™). Taken together VLPs represent a potential strategy for the fight against new pandemic influenza viruses.
登录
查看更多内容
影响因子：
5.4
作者：
Perrone, Lucy A.;Ahmad, Attiya;Tumpey, Terrence M.
通讯作者：
Tumpey, Terrence M.
影响因子：
5.4
作者：
Quan, Fu-Shi;Huang, Chunzi;Kang, Sang-Moo
通讯作者：
Kang, Sang-Moo
影响因子：
13.8
作者：
D'Aoust, Marc-Andre;Lavoie, Pierre-Olivier;Vezina, Louis-P.
通讯作者：
Vezina, Louis-P.
影响因子：
46.9
作者：
Kost, TA;Condreay, JP;Jarvis, DL
通讯作者：
Jarvis, DL
影响因子：
4.8
作者：
Szecsi, Judit;Boson, Bertrand;Johnsson, Per;Dupeyrot-Lacas, Pia;Matrosovich, Mikhail;Klenk, Hans-Dieter;Klatzmann, David;Volchkov, Viktor;Cosset, Francois-Loic
通讯作者：
Cosset, Francois-Loic