A potent adjuvant effect of a CD1d-binding NKT cell ligand in human immune system mice.
A potent adjuvant effect of a CD1d-binding NKT cell ligand in human immune system mice.
复制标题
DOI：
10.1080/14760584.2017.1256208
复制
发表时间：
2017-01
影响因子：
6.2
通讯作者：
Tsuji M
中科院分区：
文献类型：
--
作者：
Li X;Huang J;Kaneko I;Zhang M;Iwanaga S;Yuda M;Tsuji M
一种CD1d结合的不变自然杀伤T(INKT)细胞刺激性糖脂，即7DW8-5，被证明能增强辐射减弱子孢子(RAS)疟疾疫苗在小鼠中的效力。在目前的研究中，我们的目标是确定7DW8-5是否能够在人类免疫系统(HIS)小鼠中显示出强大的佐剂作用。His-A2/hCD1d小鼠是由携带编码人类CD1d分子和人类白细胞抗原A*0201基因的腺相关病毒血清9型NSG小鼠转导，然后植入人造血干细胞而产生的，具有功能性人iNKT细胞和CD8+T细胞。用人CD1d四聚体和人HLA-A*0201四聚体分别测定His-A2/hCD1d小鼠体内人iNKT细胞对7DW8-5和人类白细胞抗原A*0201限制性的人CD8+T细胞对人疟疾抗原的反应量。我们发现，7DW8-5在体外刺激His-A2/hCD1d小鼠和His-A2/hCD1d小鼠来源的人iNKT细胞。我们还发现，7DW8-5显著增加了His-A2/hCD1d小鼠体内人类疟疾抗原特异性HLA-A*0201限制性的人CD8+T细胞反应水平。我们的研究表明，7DW8-5可以通过增强疟疾特异性的人CD8+T细胞应答而对RAS疫苗诱导的抗疟疾免疫表现出强大的佐剂作用。
A CD1d-binding invariant natural killer T (iNKT)-cell stimulatory glycolipid, namely 7DW8–5, is shown to enhance the efficacy of radiation-attenuated sporozoites (RAS)-based malaria vaccine in mice. In the current study, we aim to determine whether 7DW8-5 can display a potent adjuvant effect in human immune system (HIS) mice. HIS-A2/hCD1d mice, which possess both functional human iNKT cells and CD8+ T cells, were generated by the transduction of NSG mice with adeno-associated virus serotype 9 expressing genes that encode human CD1d molecules and HLA-A*0201, followed by the engraftment of human hematopoietic stem cells. The magnitudes of human iNKT-cell response against 7DW8-5 and HLA-A*0201-restricted human CD8+ T-cell response against a human malaria antigen in HIS-A2/hCD1d mice were determined by using human CD1d tetramer and human HLA-A*0201 tetramer, respectively. We found that 7DW8-5 stimulates human iNKT cells in HIS-A2/hCD1d mice, as well as those derived from HIS-A2/hCD1d mice in vitro. We also found that 7DW8-5 significantly increases the level of a human malarial antigen-specific HLA-A*0201-restricted human CD8+ T-cell response in HIS-A2/hCD1d mice. Our study indicates that 7DW8-5 can display a potent adjuvant effect on RAS vaccine-induced anti-malarial immunity by augmenting malaria-specific human CD8+ T-cell response.
登录
查看更多内容
影响因子：
15.9
作者：
Crompton, Peter D.;Pierce, Susan K.;Miller, Louis H.
通讯作者：
Miller, Louis H.
影响因子：
2.2
作者：
Liu, Yang;Goff, Randal D.;Savage, Paul B.
通讯作者：
Savage, Paul B.
DOI：
10.1016/j.clim.2010.11.009
发表时间：
2011-08
期刊：
Clinical immunology (Orlando, Fla.)
影响因子：
--
作者：
Padte NN;Li X;Tsuji M;Vasan S
通讯作者：
Vasan S
影响因子：
4.4
作者：
Ko, SY;Ko, HJ;Kang, CY
通讯作者：
Kang, CY
影响因子：
2.7
作者：
Lepus, Christin M.;Gibson, Thomas F.;Gerber, Scott A.;Kawikova, Ivana;Szczepanik, Marian;Hossain, Jaber;Ablamunits, Vitaly;Kirkiles-Smith, Nancy;Herold, Kevan C.;Donis, Ruben O.;Bothwell, Alfred L.;Pober, Jordan S.;Harding, Martha J.
通讯作者：
Harding, Martha J.