Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
复制标题
DOI：
10.1038/s41419-018-0519-8
复制
发表时间：
2018-05-01
影响因子：
9
通讯作者：
Shyu WC
中科院分区：
文献类型：
--
作者：
Fan JR;Lee HT;Lee W;Lin CH;Hsu CY;Hsieh CH;Shyu WC
成人嗅觉粘膜是一种高度再生的组织，具有独特的终身神经发生能力，被认为含有naïve但严格控制的干细胞群。它将为各种基于干细胞的治疗提供独特的益处，例如中风治疗。在这里，我们鉴定了一个由CBX7表观遗传抑制因子调节的成体多能样嗅觉干细胞（APOSCs）亚群。APOSCs形成一个浮球，表达多能性标志物Nanog、Oct-4、Sox-2和SSEA-4，并显示碱性磷酸酶活性。此外，APOSCs表现出自我更新和多能分化成所有三种胚层的潜能。此外，aposc与CBX7共表达多能性标记。在其自然生态位内，CBX7+/+小鼠的APOSCs对自发或损伤诱导的组织再生均迅速作出反应。然而，CBX7−/−小鼠的APOSCs表现出自我更新和分化潜力受损。同样，体外培养的CBX7−/−APOSCs也会过早衰老，而CBX7+/+ APOSCs仍在积极分裂，这表明CBX7是APOSCs自我更新所必需的。脑内植入APOSCs可改善脑卒中介导的神经功能障碍。这些发现表明CBX7在APOSCs的再生特性中起着关键作用，并表明自体APOSCs植入脑卒中治疗的安全性和可行性。
The adult olfactory mucosa, a highly regenerative tissue with unique life-long neurogenesis ability, is thought to harbor a naïve yet tightly controlled stem cell population. It will provide unique benefits in various stem cell-based therapies, such as stroke treatment. Here, we identified a subpopulation of adult pluripotent-like olfactory stem cells (APOSCs), which were modulated by an epigenetic repressor of CBX7. APOSCs form a floating sphere, express pluripotency markers Nanog, Oct-4, Sox-2, and SSEA-4 and show alkaline phosphatase activity. In addition, APOSCs display self-renewal and a pluripotent potential to differentiate into all three germ layers. Moreover, APOSCs coexpress pluripotency markers with CBX7. Within their natural niche, APOSCs from CBX7+/+ mice responded promptly to either spontaneous or injury-induced tissue regeneration. However, APOSCs from CBX7−/− mice manifested an impaired self-renewal and differentiation potential. Similarly, in vitro-cultivated CBX7−/− APOSCs underwent premature senescence, whereas CBX7+/+ APOSCs still actively divided, indicating that CBX7 is required for the self-renewal of APOSCs. Intracerebral implantation of APOSCs improved the stroke-mediated neurological dysfunction in rodents. These findings indicate that CBX7 plays a critical role in the regenerative properties of APOSCs and indicate the safety and feasibility of implantation of autologous APOSCs in stroke treatment.
登录
查看更多内容
应激诱导蛋白-1在募集骨髓细胞中的作用，进入缺血性大脑。
影响因子：
11.1
作者：
Lee, Shin-Da;Lai, Ted Weita;Lin, Shinn-Zong;Lin, Chen-Huan;Hsu, Yung-Hsiang;Li, Chi-Yuan;Wang, Hsiao-Jung;Lee, Wei;Su, Ching-Yuan;Yu, Yung-Luen;Shyu, Woei-Cherng
通讯作者：
Shyu, Woei-Cherng
影响因子：
--
作者：
Karussis, Dimitrios;Karageorgiou, Clementine;Vaknin-Dembinsky, Adi;Gowda-Kurkalli, Basan;Gomori, John M.;Kassis, Ibrahim;Bulte, Jeff W. M.;Petrou, Panayiota;Ben-Hur, Tamir;Abramsky, Oded;Slavin, Shimon
通讯作者：
Slavin, Shimon
影响因子：
50.3
作者：
Gargiulo, Gaetano;Cesaroni, Matteo;van Lohuizen, Maarten
通讯作者：
van Lohuizen, Maarten
影响因子：
5.3
作者：
Abdouh, Mohamed;Facchino, Sabrina;Bernier, Gilbert
通讯作者：
Bernier, Gilbert
影响因子：
4.8
作者：
Kaltschmidt, Barbara;Kaltschmidt, Christian;Widera, Darius
通讯作者：
Widera, Darius