VaCRK2 Mediates Gray Mold Resistance in Vitis amurensis by Activating the Jasmonate Signaling Pathway
VaCRK2 Mediates Gray Mold Resistance in Vitis amurensis by Activating the Jasmonate Signaling Pathway
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VaCRK2 通过激活茉莉酸信号通路介导山葡萄灰霉病抗性
DOI：
10.3390/agronomy11081672
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Dongying Gong
中科院分区：
文献类型：
--
作者：
Tinggang Li;Huanhuan Gao;Xiaoning Tang;Dongying Gong
富含半胱氨酸的受体样激酶（CRKs）是植物中普遍存在的一类受体样激酶，在植物抗病过程中起着重要作用。灰霉病是由灰葡萄孢菌（Botrytis cinerea）引起的一种重要葡萄病害。然而，CRK基因及其在葡萄灰霉病抗性中的功能尚未阐明。本研究旨在鉴定和表征葡萄中的CRKs，并确定其在灰霉病抗性中的作用。从葡萄属山葡萄中鉴定出4个CRK，根据其基因组分布将其命名为VaCRK 1-VaCRK 4。在拟南芥中异位表达这4种VaCRKs，研究其在抗B防御反应中的功能。灰色区域。VaCRK 2在A.拟南芥对B具有抗性。灰色区域。B处理后，VaCRK 2在抗灰霉病葡萄品种中的表达量显著增加。灰葡萄接种和茉莉酸甲酯处理。此外，还研究了茉莉酸（JA）信号通路相关基因在VaCRK 2过表达的A. B处理后，拟南芥的生长量显著增加。灰霉病接种，导致病程相关（PR）基因的上调和活性氧（ROS）的积累。总之，这些结果表明VaCRK 2赋予对B的抗性。通过JA信号通路激活PR基因表达和氧化爆发来抑制番茄红素的合成。
Cysteine-rich receptor-like kinases (CRKs) are ubiquitous plant receptor-like kinases, which play a significant role in plant disease resistance. Gray mold is an economically important disease of grapes caused by Botrytis cinerea. However, CRK genes and their function in gray mold disease resistance in grapes have not been elucidated. This study aimed to identify and characterize CRKs in grapes and determine their role in gray mold resistance. Four CRKs were identified in Vitis amurensis and named VaCRK1–VaCRK4 according to their genomic distribution. The four VaCRKs were ectopically expressed in Arabidopsis thaliana to study their function in defense response against B. cinerea. Heterologous expression of VaCRK2 in A. thaliana conferred resistance to B. cinerea. VaCRK2 expression in gray mold-resistant grape cultivar increased significantly after B. cinerea inoculation and methyl jasmonate treatment. Furthermore, the expression of jasmonic acid (JA) signaling pathway-related genes in VaCRK2 overexpression lines of A. thaliana was significantly increased after B. cinerea inoculation, leading to the upregulation of pathogenesis-related (PR) genes and reactive oxygen species (ROS) accumulation. Overall, these results suggest that VaCRK2 confers resistance to B. cinerea by activating PR gene expression and oxidative burst through the JA signaling pathway.
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DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dangl JL;Horvath DM;Staskawicz BJ
通讯作者：
Staskawicz BJ
影响因子：
14.9
作者：
Hunter S;Jones P;Mitchell A;Apweiler R;Attwood TK;Bateman A;Bernard T;Binns D;Bork P;Burge S;de Castro E;Coggill P;Corbett M;Das U;Daugherty L;Duquenne L;Finn RD;Fraser M;Gough J;Haft D;Hulo N;Kahn D;Kelly E;Letunic I;Lonsdale D;Lopez R;Madera M;Maslen J;McAnulla C;McDowall J;McMenamin C;Mi H;Mutowo-Muellenet P;Mulder N;Natale D;Orengo C;Pesseat S;Punta M;Quinn AF;Rivoire C;Sangrador-Vegas A;Selengut JD;Sigrist CJ;Scheremetjew M;Tate J;Thimmajanarthanan M;Thomas PD;Wu CH;Yeats C;Yong SY
通讯作者：
Yong SY
影响因子：
5.1
作者：
Chen, KG;Du, LQ;Chen, ZX
通讯作者：
Chen, ZX
影响因子：
4.9
作者：
Gu, Jia;Sun, Jiawei;Wang, Dongmei
通讯作者：
Wang, Dongmei
DOI：
10.1016/j.bbrc.2014.02.013
发表时间：
2014-03-07
影响因子：
3.1
作者：
Idanheimo, Niina;Gauthier, Adrien;Wrzaczek, Michael
通讯作者：
Wrzaczek, Michael