Choline deprivation induces hyperhomocysteinemia in rats fed low methionine diets.
Choline deprivation induces hyperhomocysteinemia in rats fed low methionine diets.
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胆碱剥夺会导致喂食低蛋氨酸饮食的大鼠出现高同型半胱氨酸血症。
DOI：
10.3177/jnsv.54.483
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发表时间：
2008
影响因子：
1.6
通讯作者：
K. Sugiyama
中科院分区：
文献类型：
--
作者：
Minoru Setoue;Seiya Ohuchi;T. Morita;K. Sugiyama
为了阐明饮食胆碱水平与血浆同型半胱氨酸浓度之间的关系，在饲喂标准（25%）酪蛋白（25 C）饮食或标准大豆蛋白（25 S）饮食的大鼠中研究了胆碱剥夺对血浆同型半胱氨酸浓度和相关变量的影响。采用25 S饲料，研究了胆碱剥夺的时间依赖性效应和三种促脂剂的比较效应。喂养大鼠胆碱剥夺25 S饮食10天显着增加血浆总同型半胱氨酸浓度的水平比对照组高2.68倍，而胆碱剥夺没有影响大鼠喂养25 C饮食。肝S-腺苷高半胱氨酸和高半胱氨酸浓度增加，肝甜菜碱浓度和胱硫醚β-合成酶的活性降低，但不甜菜碱-高半胱氨酸S-甲基转移酶，脂肪肝也发生在大鼠喂养胆碱剥夺25 S饮食。血浆同型半胱氨酸浓度增加时，大鼠喂养胆碱剥夺25 S饮食只有3天，并持续增加到20天。补充甜菜碱或蛋氨酸可有效抑制胆碱剥夺引起的高同型半胱氨酸血症。胆碱剥夺引起高同型半胱氨酸血症，也在大鼠喂养胆碱剥夺低（10%）酪蛋白饮食。结果表明，胆碱剥夺可以很容易地诱导明显的高同型半胱氨酸血症时，大鼠喂养相对低蛋氨酸饮食，如标准的大豆蛋白饮食和低酪蛋白饮食，可能通过抑制同型半胱氨酸去除的再甲基化和胱硫醚形成。此高同型半胱氨酸血症可能是一个有用的模型，研究甜菜碱在调节血浆同型半胱氨酸浓度的作用。
To clarify the relationship between dietary choline level and plasma homocysteine concentration, the effects of choline deprivation on plasma homocysteine concentration and related variables were investigated in rats fed a standard (25%) casein (25C) diet or standard soybean protein (25S) diet. Using the 25S diet, the time-dependent effect of choline deprivation and the comparative effects of three kinds of lipotropes were also investigated. Feeding rats with the choline-deprived 25S diet for 10 d significantly increased plasma total homocysteine concentration to a level 2.68-times higher than that of the control group, whereas choline deprivation had no effect in rats fed the 25C diet. Increases in hepatic S-adenosylhomocysteine and homocysteine concentrations, decreases in hepatic betaine concentration and the activity of cystathionine beta-synthase, but not betaine-homocysteine S-methyltransferase, and fatty liver also occurred in rats fed the choline-deprived 25S diet. Plasma homocysteine concentration increased when rats were fed the choline-deprived 25S diet for only 3 d, and the increase persisted up to 20 d. The hyperhomocysteinemia induced by choline deprivation was effectively suppressed by betaine or methionine supplementation. Choline deprivation caused hyperhomocysteinemia also in rats fed a choline-deprived low (10%) casein diet. The results indicate that choline deprivation can easily induce prominent hyperhomocysteinemia when rats are fed relatively low methionine diets such as a standard soybean protein diet and low casein diet, possibly through the suppression of homocysteine removal by both remethylation and cystathionine formation. This hyperhomocysteinemia might be a useful model for investigating the role of betaine in the regulation of plasma homocysteine concentration.
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DOI：
10.1093/ajcn.81.2.440
发表时间：
2005-02
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
K. da Costa;Christopher E. Gaffney;L. Fischer;S. Zeisel
通讯作者：
K. da Costa;Christopher E. Gaffney;L. Fischer;S. Zeisel
DOI：
10.1016/s0899-9007(00)00349-x
发表时间：
2000-07
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
Steven;-H.;ZEISEL’;Kerry-Ann;DA Costa;Peter;-D.;Franklin;Edward;-A.;Alexander;-J.;Thomas;Lamont;Nancy;F. Sheard;Alexa;Beiser
通讯作者：
Steven;-H.;ZEISEL’;Kerry-Ann;DA Costa;Peter;-D.;Franklin;Edward;-A.;Alexander;-J.;Thomas;Lamont;Nancy;F. Sheard;Alexa;Beiser
影响因子：
7.1
作者：
Cho, E;Zeisel, SH;Willett, WC
通讯作者：
Willett, WC
影响因子：
4.2
作者：
HORNE, DW;COOK, RJ;WAGNER, C
通讯作者：
WAGNER, C