Elucidating the regulatory mechanism of Swi1 prion in global transcription and stress responses.
Elucidating the regulatory mechanism of Swi1 prion in global transcription and stress responses.
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DOI：
10.1038/s41598-020-77993-0
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发表时间：
2020-12-14
影响因子：
4.6
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Du Z;Regan J;Bartom E;Wu WS;Zhang L;Goncharoff DK;Li L
在酿酒酵母中，已鉴定的朊病毒中普遍存在转录调节因子，然而，朊病毒如何影响全基因组转录尚不清楚。我们在这里表明，朊病毒（[SWI+]）和突变体（swi 1）形式的Swi 1，SWI/SNF染色质重塑复合物的亚基，赋予显着不同的转录组学配置文件。在[SWI+]细胞中，编码34种转录因子（TF）和24种Swi 1相互作用蛋白的基因可以进行转录修饰。几种TF在[SWI+]细胞中显示增强的聚集。进一步的分析表明，这种改变是指定[SWI+]细胞转录组特征的关键因素。有趣的是，swi 1+和[SWI+]对细胞功能产生了不同的、通常相反的影响。翻译相关的活动，特别是，显着减少swi 1细胞。尽管swl+和[SWI+]细胞对热、渗透压和干旱胁迫同样敏感，但对于一组测试的化学应激物观察到有害、中性或有益的影响。进一步的分析表明，swi 1+和[SWI+]细胞的环境应激反应（ESR）机制不同，应激诱导型ESR（iESR）被[SWI+]抑制，而swi 1+则无变化，而应激抑制型ESR（rESR）被[SWI+]诱导，但被swi 1+抑制。因此，我们的工作主要通过[SWI+]的转录组修饰证明了功能获得的结果，并强调了朊病毒介导的酵母转录和表型调节。
Transcriptional regulators are prevalent among identified prions in Saccharomyces cerevisiae, however, it is unclear how prions affect genome-wide transcription. We show here that the prion ([SWI+]) and mutant (swi1∆) forms of Swi1, a subunit of the SWI/SNF chromatin-remodeling complex, confer dramatically distinct transcriptomic profiles. In [SWI+] cells, genes encoding for 34 transcription factors (TFs) and 24 Swi1-interacting proteins can undergo transcriptional modifications. Several TFs show enhanced aggregation in [SWI+] cells. Further analyses suggest that such alterations are key factors in specifying the transcriptomic signatures of [SWI+] cells. Interestingly, swi1∆ and [SWI+] impose distinct and oftentimes opposite effects on cellular functions. Translation-associated activities, in particular, are significantly reduced in swi1∆ cells. Although both swi1∆ and [SWI+] cells are similarly sensitive to thermal, osmotic and drought stresses, harmful, neutral or beneficial effects were observed for a panel of tested chemical stressors. Further analyses suggest that the environmental stress response (ESR) is mechanistically different between swi1∆ and [SWI+] cells—stress-inducible ESR (iESR) are repressed by [SWI+] but unchanged by swi1∆ while stress-repressible ESR (rESR) are induced by [SWI+] but repressed by swi1∆. Our work thus demonstrates primarily gain-of-function outcomes through transcriptomic modifications by [SWI+] and highlights a prion-mediated regulation of transcription and phenotypes in yeast.
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朊病毒样聚合是抗病毒免疫防御和炎症小体激活中信号转导的基础。
影响因子：
64.5
作者：
Cai X;Chen J;Xu H;Liu S;Jiang QX;Halfmann R;Chen ZJ
通讯作者：
Chen ZJ
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
新发现的朊病毒与酿酒酵母中染色质重塑因子 Swi1 相关
影响因子：
30.8
作者：
Du, Zhiqiang;Park, Kyung-Won;Li, Liming
通讯作者：
Li, Liming
影响因子：
8.6
作者：
Du, Zhiqiang;Valtierra, Stephanie;Li, Liming
通讯作者：
Li, Liming
影响因子：
3.3
作者：
Du, Zhiqiang;Li, Liming
通讯作者：
Li, Liming