B-box containing protein 1 from Malus domestica (MdBBX1) is involved in the abiotic stress response.
B-box containing protein 1 from Malus domestica (MdBBX1) is involved in the abiotic stress response.
复制标题
含有来自苹果 (MdBBX1) 的蛋白 1 的 B 盒参与非生物胁迫反应
作者：
Dai Y;Lu Y;Zhou Z;Wang X;Ge H;Sun Q
B-box蛋白（BBXs）作为转录因子，主要调控光形态建成。然而，植物BBXs响应非生物胁迫的分子功能仍不清楚。在本研究中，我们发现一个BBX从苹果（MdBBX 1）参与各种非生物胁迫的反应。MdBBX 1的表达在非生物胁迫和脱落酸（阿坝）的反应显着上调。重组MdBBX 1增加大肠杆菌细胞的应激耐受性。此外，MdBBX 1在拟南芥中的过表达降低了对外源阿坝的敏感性，导致发芽率和根长分别比野生型（WT）植物更大和更长。此外，在阿坝处理下，ABI 5的表达在MdBBX 1过表达系中降低。在盐胁迫和干旱胁迫下，与野生型相比，转MdBBX 1基因植株表现出更强的耐盐性和更高的存活率。此外，盐胁迫下，过量表达MdBBX 1的植株体内脯氨酸（PRO）含量增加，丙二醛（MDA）含量降低，抗氧化酶（超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX））活性增加，活性氧（ROS）积累减少。总体而言，我们的研究结果提供了证据，MdBBX 1可能通过减少ROS的产生在非生物胁迫耐受性的调节中发挥关键作用。
B-box proteins (BBXs), which act as transcription factors, mainly regulate photomorphogenesis. However, the molecular functions underlying the activity of plant BBXs in response to abiotic stress remain largely unclear. In this investigation, we found that a BBX from Malus domestica (MdBBX1) was involved in the response to various abiotic stresses. The expression of MdBBX1 was significantly upregulated in response to abiotic stresses and abscisic acid (ABA). Recombinant MdBBX1 increased stress tolerance in Escherichia coli cells. In addition, overexpression of MdBBX1 in Arabidopsis decreased sensitivity to exogenous ABA, resulting in a germination rate and root length that were greater and longer, respectively, than those of wild-type (WT) plants. Moreover, the expression of ABI5 was decreased in MdBBX1-overexpressing lines under ABA treatment. After salt and drought treatments, compared with the WT plants, the MdBBX1 transgenic plants displayed enhanced tolerance and had a higher survival rate. Furthermore, under salt stress, increased proline (PRO) contents, decreased levels of malondialdehyde (MDA), increased activity of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX)) and decreased accumulation of reactive oxygen species (ROS) were observed in the MdBBX1-overexpressing plants. Overall, our results provide evidence that MdBBX1 might play a critical role in the regulation of abiotic stress tolerance by reducing the generation of ROS.
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