Sequence analysis of the genome of carnation (Dianthus caryophyllus L.).
Sequence analysis of the genome of carnation (Dianthus caryophyllus L.).
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DOI：
10.1093/dnares/dst053
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Tabata S
中科院分区：
文献类型：
--
作者：
Yagi M;Kosugi S;Hirakawa H;Ohmiya A;Tanase K;Harada T;Kishimoto K;Nakayama M;Ichimura K;Onozaki T;Yamaguchi H;Sasaki N;Miyahara T;Nishizaki Y;Ozeki Y;Nakamura N;Suzuki T;Tanaka Y;Sato S;Shirasawa K;Isobe S;Miyamura Y;Watanabe A;Nakayama S;Kishida Y;Kohara M;Tabata S
克隆了香石竹（Dianthus caryophyllus L.）CV.使用不同的新一代多重测序平台的组合来确定“Francesco”。非冗余序列的总长度为568 887 315 bp，由45 088个骨架组成，占k-mer分析估计的622 Mb香石竹基因组的91%。重叠群和支架的N50值分别为16 644 bp和60 737 bp，最长支架为1 287 144 bp。重叠群序列的平均GC含量为36%。在组装的基因组序列中，分别鉴定了总共1050、13、92和143个tRNA、rRNA、snoRNA和miRNA基因。对于蛋白质编码基因，推导出43266个完整和部分基因结构，不包括转座因子。从核心真核基因的覆盖率推断，基因覆盖率为1.98%。密集的香石竹基因的分配和与其他植物物种的比较特征的香石竹基因组。本研究结果可为香石竹的基础和应用研究，特别是新品种的选育提供宝贵的资源。关于基因组序列的进一步信息可在.
The whole-genome sequence of carnation (Dianthus caryophyllus L.) cv. ‘Francesco’ was determined using a combination of different new-generation multiplex sequencing platforms. The total length of the non-redundant sequences was 568 887 315 bp, consisting of 45 088 scaffolds, which covered 91% of the 622 Mb carnation genome estimated by k-mer analysis. The N50 values of contigs and scaffolds were 16 644 bp and 60 737 bp, respectively, and the longest scaffold was 1 287 144 bp. The average GC content of the contig sequences was 36%. A total of 1050, 13, 92 and 143 genes for tRNAs, rRNAs, snoRNA and miRNA, respectively, were identified in the assembled genomic sequences. For protein-encoding genes, 43 266 complete and partial gene structures excluding those in transposable elements were deduced. Gene coverage was ∼98%, as deduced from the coverage of the core eukaryotic genes. Intensive characterization of the assigned carnation genes and comparison with those of other plant species revealed characteristic features of the carnation genome. The results of this study will serve as a valuable resource for fundamental and applied research of carnation, especially for breeding new carnation varieties. Further information on the genomic sequences is available at .
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影响因子：
6.9
作者：
Hoeberichts, Frank A.;van Doorn, Wouter G.;van Wordragen, Monique F.
通讯作者：
van Wordragen, Monique F.
影响因子：
7.4
作者：
Christinet, L;Burdet, FRX;Zryd, JP
通讯作者：
Zryd, JP
影响因子：
4.3
作者：
Ichimura, K;Kohata, K;Ito, A
通讯作者：
Ito, A
DOI：
10.2503/jjshs1.80.486
发表时间：
2011-10-01
影响因子：
--
作者：
Onozaki, Takashi;Yagi, Masafumi;Shibata, Michio
通讯作者：
Shibata, Michio
影响因子：
3.7
作者：
Peng Z;Zhao Z;Nath N;Froula JL;Clum A;Zhang T;Cheng JF;Copeland AC;Pennacchio LA;Chen F
通讯作者：
Chen F