Recruitment of Polo kinase to the spindle midzone during cytokinesis requires the Feo/Klp3A complex.
Recruitment of Polo kinase to the spindle midzone during cytokinesis requires the Feo/Klp3A complex.
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DOI：
10.1371/journal.pone.0000572
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发表时间：
2007-06-27
期刊：
影响因子：
3.7
通讯作者：
Glover DM
中科院分区：
文献类型：
--
作者：
D'Avino PP;Archambault V;Przewloka MR;Zhang W;Lilley KS;Laue E;Glover DM
Polo样激酶控制细胞分裂过程中的多个事件，包括有丝分裂进入、中心体组织、纺锤体形成、染色体分离和胞质分裂。然而，它们在胞质分裂过程中的作用还没有得到很好的理解，因为在有丝分裂的早期阶段对这些激酶的需求使得后期开始后对其功能的研究变得复杂。我们使用延时显微镜来分析果蝇组织培养细胞有丝分裂过程中波罗：：GFP的动态。后期开始后，波罗：：GFP集中在纺锤体中间区，但也扩散沿着整个长度的中央纺锤体。使用RNA干扰，我们证明，微管相关蛋白FeO和Klp 3A所需的波罗招聘到纺锤体中间区，但不是驱动蛋白帕瓦罗蒂以前认为。此外，我们表明，FeO和Klp 3A形成一个复杂的，波罗co-localizes与这两种蛋白质在胞质分裂。我们的研究结果表明，FeO/Klp 3A复合物是必要的波罗招聘到纺锤体中间区。最近在哺乳动物细胞中也有类似的发现，表明这种基本机制在进化过程中一直保持不变，尽管存在一些差异。最后，由于feo RNAi后卵裂沟的形成和侵入不受影响，我们的数据表明，波罗募集到中央纺锤体不是开沟所需的，而是胞质分裂的一些其他方面。
Polo-like kinases control multiple events during cell division, including mitotic entry, centrosome organization, spindle formation, chromosome segregation and cytokinesis. Their roles during cytokinesis, however, are not well understood because the requirement of these kinases during early stages of mitosis complicates the study of their functions after anaphase onset. We used time-lapse microscopy to analyze the dynamics of Polo::GFP in Drosophila tissue culture cells during mitosis. After anaphase onset, Polo::GFP concentrated at the spindle midzone, but also diffused along the entire length of the central spindle. Using RNA interference we demonstrate that the microtubule-associated proteins Feo and Klp3A are required for Polo recruitment to the spindle midzone, but not the kinesin Pavarotti as previously thought. Moreover, we show that Feo and Klp3A form a complex and that Polo co-localizes with both proteins during cytokinesis. Our results reveal that the Feo/Klp3A complex is necessary for Polo recruitment to the spindle midzone. A similar finding has also been recently reported in mammalian cells, suggesting that this basic mechanism has been conserved during evolution, albeit with some differences. Finally, since cleavage furrow formation and ingression are unaffected following feo RNAi, our data imply that Polo recruitment to the central spindle is not required for furrowing, but some other aspect of cytokinesis.
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影响因子：
3.7
作者：
Brennan IM;Peters U;Kapoor TM;Straight AF
通讯作者：
Straight AF
DOI：
10.1073/pnas.0701140104
发表时间：
2007-03-13
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