Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats.
Dissection of Pelvic Autonomic Ganglia and Associated Nerves in Male and Female Rats.
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DOI：
10.3791/60904
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发表时间：
2020-03-07
影响因子：
1.2
通讯作者：
Keast, Janet R.
中科院分区：
文献类型：
--
作者：
Bertrand, Martin M.;Keast, Janet R.
关键词：
双侧骨盆大神经节（MPG;同义词，骨盆神经节）是支配啮齿动物骨盆器官的节后交感神经元和副交感神经元的主要来源;人类中功能等同的结构是下腹下丛。骨盆大神经节也提供了腰骶感觉轴突到达骨盆器官的途径。这些复杂的混合神经节可以证明具有挑战性的识别和解剖的正常自主机制的进一步实验研究或建立疾病，损伤或内脏疼痛的临床前模型。在这里，我们描述了一个协议，以访问和可视化这些神经节及其相关的神经束。我们提供了雄性和雌性大鼠的示意图，因为性别之间的神经节大小和识别标志不同。该方案描述了用于体外研究的神经节的去除，但是该方法可以被整合到用于实验性干预的手术恢复方案中（例如，神经挤压、神经切除）或用于映射神经元回路（例如，通过神经示踪剂的显微注射）。我们还展示了神经节及其相关神经的主要结构后立即解剖和免疫组化染色。主要的盆神经节含有支配盆腔器官的副交感神经元和交感神经元。在这里，我们描述了一种解剖方法，并提供了识别这些神经节及其相关神经的示意图。这些方法可应用于这些神经节的体内实验操作或死后切除以作进一步研究。
The bilateral major pelvic ganglia (MPG; synonym, pelvic ganglia) are the primary source of postganglionic sympathetic and parasympathetic neurons innervating pelvic organs of rodents; the functionally equivalent structure in humans is the inferior hypogastric plexus. The major pelvic ganglia also provide the route by which lumbar and sacral sensory axons reach the pelvic organs. These complex, mixed ganglia can prove challenging to identify and dissect for further experimental study of normal autonomic mechanisms or to establish pre-clinical models of disease, injury or visceral pain. Here we describe a protocol to access and visualize these ganglia and their associated nerve tracts. We provide this protocol with schematics for both male and female rats, as the ganglion size and landmarks for identification differ between sexes. The protocol describes removal of the ganglion for in vitro study, but this method can be integrated into a surgical recovery protocol for experimental interventions (e.g., nerve crush, nerve resection) or for mapping neuronal circuits (e.g., by microinjection of neural tracers). We also demonstrate the primary structures of the ganglion and its associated nerves immediately following dissection and following immunohistochemical staining. The major pelvic ganglia contain parasympathetic and sympathetic neurons that innervate pelvic organs. Here we describe a dissection method and provide schematics for identification of these ganglia and their associated nerves. These methods can be applied to experimental manipulation of these ganglia in vivo or removal post-mortem for further study.
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影响因子：
2.5
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