Characterization of an entomopathogenic fungi target integument protein, Bombyx mori single domain von Willebrand factor type C, in the silkworm, Bombyx mori

Characterization of an entomopathogenic fungi target integument protein, Bombyx mori single domain von Willebrand factor type C, in the silkworm, Bombyx mori
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家蚕中昆虫病原真菌靶外皮蛋白 Bombyx mori 单域 von Willebrand 因子 C 型的表征

DOI:
10.1111/imb.12293
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发表时间:
2017-06
影响因子:
2.6
通讯作者:
Erjun Ling
Erjun Ling
中科院分区:
农林科学2区
文献类型:
--
作者:
Fang Han;Anrui Lu;Yi Yuan;Wuren Huang;Brenda T. Beerntsen;Junyi Huang;Erjun Ling

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昆虫表皮是保护昆虫免受病原体感染和水分蒸发的第一道防线。然而,旧的角质层必须脱落才能进入下一个发育阶段。在每次蜕皮过程中,蜕皮液产生并分泌到新旧角质层之间的区域。在以前的研究中,蛋白质家蚕单域冯维勒布兰德因子C型(BmSVWC; BGIBMGA 011399)被确定在蜕皮液波。桑和证明调节蜕皮。在这项研究中,我们表明,在波。家蚕幼虫的BmSVWC主要分布于珠被(表皮细胞和角质层)、翅盘和头部。在蜕皮阶段,BmSVWC被释放到蜕皮液中,然后在蜕皮后由表皮细胞再次产生。结果表明,真菌感染可降低角质层中BmSVWC的含量,表明BmSVWC是昆虫病原真菌的靶蛋白。因此,BmSVWC主要参与维持珠被结构的完整性,其用于保护昆虫免受物理损伤和病原体感染。
The insect cuticle works as the first line of defence to protect insects from pathogenic infections and water evaporation. However, the old cuticle must be shed in order to enter the next developmental stage. During each ecdysis, moulting fluids are produced and secreted into the area among the old and new cuticles. In a previous study, the protein Bombyx mori single domain von Willebrand factor type C (BmSVWC; BGIBMGA011399) was identified in the moulting fluids of Bo. mori and demonstrated to regulate ecdysis. In this study we show that in Bo. mori larvae, BmSVWC primarily locates to the integument (epidermal cells and cuticle), wing discs and head. During the moulting stage, BmSVWC is released into the moulting fluids, and is then produced again by epidermal cells after ecdysis. Fungal infection was shown to decrease the amount of BmSVWC in the cuticle, which indicates that BmSVWC is a target protein of entomopathogenic fungi. Thus, BmSVWC is mainly involved in maintaining the integrity of the integument structure, which serves to protect insects from physical damage and pathogenic infection.
昆虫后肠先天免疫和通过黑色化调节粪便微生物群
DOI: 10.1074/jbc.m112.354548
发表时间: 2012-04-20
影响因子: 4.8
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DOI: 10.1074/jbc.m114.599597
发表时间: 2014-11
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作者:
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发表时间: 2013-06-04
影响因子: 11.1
作者:
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通讯作者: Schlenke, Todd A.