High throughput profiling of the cotton bollworm Helicoverpa armigera immunotranscriptome during the fungal and bacterial infections.
High throughput profiling of the cotton bollworm Helicoverpa armigera immunotranscriptome during the fungal and bacterial infections.
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棉铃虫真菌和细菌感染过程中棉铃虫免疫转录组的高通量分析
DOI：
10.1186/s12864-015-1509-1
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发表时间：
2015-04-18
期刊：
影响因子：
4.4
通讯作者：
Zou Z
中科院分区：
文献类型：
--
作者：
Xiong GH;Xing LS;Lin Z;Saha TT;Wang C;Jiang H;Zou Z
在无脊椎动物中，先天免疫对于抵御入侵的病原体至关重要。棉铃虫（Helicoverpa armigera, h<s:1> bner）是最具破坏性的鳞翅目害虫之一，在世界范围内给农业生产造成巨大的经济损失。这种昆虫的免疫系统的组成部分在很大程度上是未知的。昆虫病原体的应用被认为是化学杀虫剂的替代品。然而，很少有研究关注害虫与病原菌相互作用的分子机制。在此，我们研究了棉铃虫幼虫的免疫转录组，并检测了病原体感染后基因表达的变化。该研究为棉铃虫幼虫潜在的免疫相关基因和途径提供了新的见解。本研究采用高通量RNA-seq方法测定注射缓冲液的棉蚜幼虫、真菌白僵菌或革兰氏阴性阴沟肠杆菌的免疫转录组。基于与已知参与其他昆虫免疫应答的同源序列的相似性，我们确定了编码模式识别受体、信号调节剂、免疫效应器以及Toll、IMD和JAK/STAT通路的几乎所有成员的免疫相关基因。RNA-seq数据表明，一些免疫相关基因在真菌和细菌挑战的脂肪体中被激活，而其他基因在球孢白僵菌挑战的血细胞中被抑制，包括假定的IMD和JAK-STAT途径成员。细菌感染提高了脂肪体中识别和调节基因以及血细胞中信号通路基因的表达。虽然脂肪体和血细胞都是参与免疫应答的重要器官，但我们的转录组分析显示，脂肪体中诱导的免疫相关基因多于血细胞。此外，实时荧光定量PCR分析证实，与RNA-seq数据一致，球孢酵母感染后，脂肪体中PGRP-SA1、Serpin1、Toll-14和Spz2基因的转录丰度升高，而血细胞中防御素、moricin1和gloverin1的mRNA水平上调。本研究对非模式鳞翅目害虫的宿主对真菌和细菌感染的防御进行了全球调查。获得了棉铃虫免疫相关基因的完整序列资源和表达谱。该研究为今后对该害虫的功能研究和开发特异性有效的防治剂提供了有价值的信息。本文的在线版本（doi:10.1186/s12864-015-1509-1）包含补充材料，授权用户可以使用。
Innate immunity is essential in defending against invading pathogens in invertebrates. The cotton bollworm, Helicoverpa armigera (Hübner) is one of the most destructive lepidopteran pests, which causes enormous economic losses in agricultural production worldwide. The components of the immune system are largely unknown in this insect. The application of entomopathogens is considered as an alternative to the chemical insecticides for its control. However, few studies have focused on the molecular mechanisms of host-pathogen interactions between pest insects and their pathogens. Here, we investigated the immunotranscriptome of H. armigera larvae and examined gene expression changes after pathogen infections. This study provided insights into the potential immunity-related genes and pathways in H. armigera larvae. Here, we adopted a high throughput RNA-seq approach to determine the immunotranscriptome of H. armigera larvae injected with buffer, fungal pathogen Beauveria bassiana, or Gram-negative bacterium Enterobacter cloacae. Based on sequence similarity to those homologs known to participate in immune responses in other insects, we identified immunity-related genes encoding pattern recognition receptors, signal modulators, immune effectors, and nearly all members of the Toll, IMD and JAK/STAT pathways. The RNA-seq data indicated that some immunity-related genes were activated in fungus- and bacterium-challenged fat body while others were suppressed in B. bassiana challenged hemocytes, including the putative IMD and JAK-STAT pathway members. Bacterial infection elevated the expression of recognition and modulator genes in the fat body and signal pathway genes in hemocytes. Although fat body and hemocytes both are important organs involved in the immune response, our transcriptome analysis revealed that more immunity-related genes were induced in the fat body than that hemocytes. Furthermore, quantitative real-time PCR analysis confirmed that, consistent with the RNA-seq data, the transcript abundances of putative PGRP-SA1, Serpin1, Toll-14, and Spz2 genes were elevated in fat body upon B. bassiana infection, while the mRNA levels of defensin, moricin1, and gloverin1 were up-regulated in hemocytes. In this study, a global survey of the host defense against fungal and bacterial infection was performed on the non-model lepidopteran pest species. The comprehensive sequence resource and expression profiles of the immunity-related genes in H. armigera are acquired. This study provided valuable information for future functional investigations as well as development of specific and effective agents to control this pest. The online version of this article (doi:10.1186/s12864-015-1509-1) contains supplementary material, which is available to authorized users.
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DOI：
10.1126/science.1193162
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bartholomay LC;Waterhouse RM;Mayhew GF;Campbell CL;Michel K;Zou Z;Ramirez JL;Das S;Alvarez K;Arensburger P;Bryant B;Chapman SB;Dong Y;Erickson SM;Karunaratne SH;Kokoza V;Kodira CD;Pignatelli P;Shin SW;Vanlandingham DL;Atkinson PW;Birren B;Christophides GK;Clem RJ;Hemingway J;Higgs S;Megy K;Ranson H;Zdobnov EM;Raikhel AS;Christensen BM;Dimopoulos G;Muskavitch MA
通讯作者：
Muskavitch MA
影响因子：
11.8
作者：
Boutros, M;Agaisse, H;Perrimon, N
通讯作者：
Perrimon, N
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC
影响因子：
3.7
作者：
Chen S;Yao H;Han J;Liu C;Song J;Shi L;Zhu Y;Ma X;Gao T;Pang X;Luo K;Li Y;Li X;Jia X;Lin Y;Leon C
通讯作者：
Leon C
影响因子：
2.9
作者：
Kanost, MR
通讯作者：
Kanost, MR