In Vivo Cellular Imaging for Translational Medical Research.
In Vivo Cellular Imaging for Translational Medical Research.
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DOI：
10.2174/157340509787354697
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发表时间：
2009-02-01
影响因子：
--
通讯作者：
Frank JA
中科院分区：
文献类型：
--
作者：
Arbab AS;Janic B;Haller J;Pawelczyk E;Liu W;Frank JA
使用经过修饰或基因工程改造的干细胞进行个性化治疗正在医学领域成为现实，其中同种异体或自体细胞可用于治疗，并可能用于疾病的早期诊断。造血干细胞、基质干细胞和器官特异性干细胞正在评估用于心脏、神经系统、自身免疫和其他疾病的细胞疗法。细胞毒性或基因改造的 T 细胞正在接受临床试验，用于治疗造血系统或其他恶性疾病。在临床试验中使用干细胞之前，实验动物模型中的转化研究至关重要，重点是开发非侵入性方法来跟踪这些细胞在时间和空间上归巢到目标组织。此外，有必要确定移植细胞的植入效率和功能能力。各种体内成像方式用于追踪所施用细胞的运动和掺入。用报告基因、荧光染料或不同的造影剂标记细胞，将它们转化为细胞探针或成像剂。最近的报告表明，磁性标记的细胞可以用作细胞磁共振成像（MRI）探针，展示细胞向目标组织的运输。在这篇综述中，我们将讨论将细胞转化为体内成像探针的方法，及其优点和缺点，以及细胞成像方法和相应成像方式的未来临床应用。
Personalized treatment using stem, modified or genetically engineered, cells is becoming a reality in the field of medicine, in which allogenic or autologous cells can be used for treatment and possibly for early diagnosis of diseases. Hematopoietic, stromal and organ specific stem cells are under evaluation for cell-based therapies for cardiac, neurological, autoimmune and other disorders. Cytotoxic or genetically altered T-cells are under clinical trial for the treatment of hematopoietic or other malignant diseases. Before using stem cells in clinical trials, translational research in experimental animal models are essential, with a critical emphasis on developing noninvasive methods for tracking the temporal and spatial homing of these cells to target tissues. Moreover, it is necessary to determine the transplanted cell’s engraftment efficiency and functional capability. Various in vivo imaging modalities are in use to track the movement and incorporation of administered cells. Tagging cells with reporter genes, fluorescent dyes or different contrast agents transforms them into cellular probes or imaging agents. Recent reports have shown that magnetically labeled cells can be used as cellular magnetic resonance imaging (MRI) probes, demonstrating the cell trafficking to target tissues. In this review, we will discuss the methods to transform cells into probes for in vivo imaging, along with their advantages and disadvantages as well as the future clinical applicability of cellular imaging method and corresponding imaging modality.
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影响因子：
19.7
作者：
Arbab, AS;Bashaw, LA;Frank, JA
通讯作者：
Frank, JA
影响因子：
5.4
作者：
Baumjohann, Dirk;Hess, Andreas;Lutz, Manfred B.
通讯作者：
Lutz, Manfred B.
影响因子：
20.3
作者：
Berger, C;Flowers, ME;Riddell, SR
通讯作者：
Riddell, SR
影响因子：
82.9
作者：
Benedetti, S;Pirola, B;Finocchiaro, G
通讯作者：
Finocchiaro, G
影响因子：
15.9
作者：
Aboody, KS;Najbauer, J;Perides, G
通讯作者：
Perides, G