Essential structural and functional roles of the Cmr4 subunit in RNA cleavage by the Cmr CRISPR-Cas complex.
Essential structural and functional roles of the Cmr4 subunit in RNA cleavage by the Cmr CRISPR-Cas complex.
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DOI：
10.1016/j.celrep.2014.11.007
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发表时间：
2014-12-11
期刊：
影响因子：
8.8
通讯作者：
Stagg SM
中科院分区：
文献类型：
--
作者：
Ramia NF;Spilman M;Tang L;Shao Y;Elmore J;Hale C;Cocozaki A;Bhattacharya N;Terns RM;Terns MP;Li H;Stagg SM
The Cmr complex is the multi-subunit effector complex of the Type III-B CRISPR-Cas immune system. The Cmr complex recognizes a target RNA through base pairing with the integral CRISPR RNA (crRNA) and cleaves the target at multiple regularly spaced locations within the complementary region. To understand the molecular basis of the function of this complex, we have assembled information from electron microscopic and x-ray crystallographic structural studies and mutagenesis of a complete Pyrococcus furiosus Cmr complex. Our findings reveal that four helically-packed Cmr4 subunits, which comprise the backbone of the Cmr complex, act as a platform to support crRNA binding and target RNA cleavage. Interestingly, we found a hook-like structural feature associated with Cmr4 that is likely the site of target RNA binding and cleavage. Our results also elucidate analogies in the mechanisms of crRNA and target molecule binding by the distinct Cmr Type III-A and Cascade Type I-E complexes.
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影响因子：
16
作者：
Hale, Caryn R.;Majumdar, Sonali;Elmore, Joshua;Pfister, Neil;Compton, Mark;Olson, Sara;Resch, Alissa M.;Glover, Claiborne V. C., III;Graveley, Brenton R.;Terns, Rebecca M.;Terns, Michael P.
通讯作者：
Terns, Michael P.
影响因子：
16
作者：
Spilman M;Cocozaki A;Hale C;Shao Y;Ramia N;Terns R;Terns M;Li H;Stagg S
通讯作者：
Stagg S
影响因子：
16
作者：
Rouillon, Christophe;Zhou, Min;Zhang, Jing;Politis, Argyris;Beilsten-Edmands, Victoria;Cannone, Giuseppe;Graham, Shirley;Robinson, Carol V.;Spagnolo, Laura;White, Malcolm F.
通讯作者：
White, Malcolm F.
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
DOI：
10.1126/science.1159689
发表时间：
2008-08-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Brouns SJ;Jore MM;Lundgren M;Westra ER;Slijkhuis RJ;Snijders AP;Dickman MJ;Makarova KS;Koonin EV;van der Oost J
通讯作者：
van der Oost J