Structure of the CRISPR interference complex CSM reveals key similarities with cascade.
Structure of the CRISPR interference complex CSM reveals key similarities with cascade.
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DOI：
10.1016/j.molcel.2013.08.020
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发表时间：
2013-10-10
期刊：
影响因子：
16
通讯作者：
White, Malcolm F.
中科院分区：
文献类型：
--
作者：
Rouillon, Christophe;Zhou, Min;Zhang, Jing;Politis, Argyris;Beilsten-Edmands, Victoria;Cannone, Giuseppe;Graham, Shirley;Robinson, Carol V.;Spagnolo, Laura;White, Malcolm F.
规则间隔回文重复序列（CRISPR）系统是原核生物中的适应性免疫系统。由CRISPR相关（cas）基因编码的干扰复合物利用小RNA进行同源性定向检测和随后降解入侵的遗传元件，并且它们已被分为三种主要类型（I-III）。III型复合物共享Cas 10亚基，但根据其对DNA或RNA靶标的特异性分别被细分为IIIA型（CSM）和IIIB型（CMR）。CSM在限制接合质粒在表皮葡萄球菌中传播中的作用于2008年首次描述。在这里，我们报告了一个详细的调查CSM复合物的组成和结构的古硫化叶菌solfataricus，使用电子显微镜，质谱和深度测序的组合。这揭示了CSM复合物的三维模型，该复合物包括一个螺旋组件，该组件令人想起I型（级联）家族的骨架结构。从硫磺硫化叶菌（Sulfolobus solfataricus）中分离出CSM复合物，并对其进行了纯化和表征。电镜观察显示CSM复合物具有螺旋骨架，与Cascade复合物具有惊人的相似性。质谱确定了CSM复合物的亚基化学计量和结构，亚基通过甲基化、乙酰化和磷酸化修饰。
The Clustered Regularly Interspaced Palindromic Repeats (CRISPR) system is an adaptive immune system in prokaryotes. Interference complexes encoded by CRISPR-associated (cas) genes utilize small RNAs for homology-directed detection and subsequent degradation of invading genetic elements, and they have been classified into three main types (I–III). Type III complexes share the Cas10 subunit but are subclassifed as type IIIA (CSM) and type IIIB (CMR), depending on their specificity for DNA or RNA targets, respectively. The role of CSM in limiting the spread of conjugative plasmids in Staphylococcus epidermidis was first described in 2008. Here, we report a detailed investigation of the composition and structure of the CSM complex from the archaeon Sulfolobus solfataricus, using a combination of electron microscopy, mass spectrometry, and deep sequencing. This reveals a three-dimensional model for the CSM complex that includes a helical component strikingly reminiscent of the backbone structure of the type I (Cascade) family. The CSM complex from Sulfolobus solfataricus has been purified and characterized EM reveals a helical backbone with striking similarities to the Cascade complex Mass spectrometry defines the subunit stoichiometry and organization of the complex CSM subunits are modified by methylation, acetylation, and phosphorylation
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影响因子：
16
作者：
Hale, Caryn R.;Majumdar, Sonali;Elmore, Joshua;Pfister, Neil;Compton, Mark;Olson, Sara;Resch, Alissa M.;Glover, Claiborne V. C., III;Graveley, Brenton R.;Terns, Rebecca M.;Terns, Michael P.
通讯作者：
Terns, Michael P.
DOI：
10.1126/science.1165771
发表时间：
2008-12-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Marraffini LA;Sontheimer EJ
通讯作者：
Sontheimer EJ
影响因子：
2
作者：
Giles, Kevin;Williams, Jonathan P.;Campuzano, Iain
通讯作者：
Campuzano, Iain
影响因子：
14.8
作者：
Hernandez, Helena;Robinson, Carol V.
通讯作者：
Robinson, Carol V.
影响因子：
5.7
作者：
Shao, Yaming;Cocozaki, Alexis I.;Li, Hong
通讯作者：
Li, Hong