Developmentally regulated mRNAs in 3T3-adipocytes: analysis of transcriptional control.
Developmentally regulated mRNAs in 3T3-adipocytes: analysis of transcriptional control.
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DOI：
10.1083/jcb.100.2.514
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发表时间：
1985-02
期刊：
影响因子：
--
通讯作者：
Spiegelman BM
中科院分区：
文献类型：
--
作者：
Cook KS;Hunt CR;Spiegelman BM
我们已经研究了mRNA合成的调节过程中3 T3脂肪细胞分化，通过测量特定基因的转录在分离的前脂肪细胞和脂肪细胞核。通过将新合成的RNA与编码甘油磷酸脱氢酶（GPD）的cDNA克隆杂交来测定转录，所述甘油磷酸脱氢酶（GPD）是13 K的诱导蛋白，其在此显示与髓鞘蛋白P-2相关，28 K的诱导蛋白，肌动蛋白，以及两种不受发育调节的RNA。GPD和13 K的转录在脂肪细胞中观察到，但在前脂肪细胞核中未观察到。肌动蛋白在两种类型的细胞核中转录，但在脂肪细胞中的水平较低。对于大多数检查的RNA，有一个一致的核转录量和脂肪细胞中相应的细胞质mRNA的丰度之间的关系。然而，13 K和28 K mRNA的丰度比仅通过其核转录预测的丰度高10-100倍。使用5，6-二氯-1-β-D-呋喃核糖基苯并咪唑抑制mRNA合成的初步mRNA周转实验表明，这些mRNA在脂肪细胞细胞质中比所检测的其他mRNA稳定得多。这些结果表明，在脂肪细胞分化过程中，特定基因的转录增加，并表明其他水平的控制，特别是mRNA稳定性，可能有助于脂肪细胞中某些发育调节mRNA的相对丰度。
We have investigated the regulation of mRNA synthesis during 3T3- adipocyte differentiation by measuring the transcription of specific genes in isolated preadipocyte and adipocyte nuclei. Transcription was assayed by hybridization of newly synthesized RNA to cDNA clones coding for glycerophosphate dehydrogenase (GPD), the induced protein of 13K which is shown here to be related to myelin protein P-2, the induced protein of 28K, actin, and two RNAs that are not developmentally regulated. Transcription of GPD and 13K was observed in adipocyte but not preadipocyte nuclei. Actin was transcribed in both types of nuclei but at a lower level in adipocytes. For most of the RNAs examined, there was a consistent relationship between amounts of nuclear transcription and the abundance of the corresponding cytoplasmic mRNA in adipocytes. However, 13K and 28K mRNAs are 10-100 times more abundant than would be predicted by their nuclear transcription alone. Preliminary mRNA turnover experiments in which 5,6-dichloro-1-beta-D- ribofuranosylbenzimidazole was used to inhibit mRNA synthesis suggest that these mRNAs are much more stable in the adipocyte cytoplasm than the other mRNAs examined. These results indicate that the transcription of specific genes is increased during adipocyte differentiation and suggest that other levels of control, particularly mRNA stability, may contribute to the relative abundance of certain developmentally- regulated mRNAs in adipocytes.
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影响因子：
64.5
作者：
HARPOLD, MM;EVANS, RM;DARNELL, JE
通讯作者：
DARNELL, JE
DOI：
10.1073/pnas.81.17.5468
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
BERNLOHR, DA;ANGUS, CW;KELLY, TJ
通讯作者：
KELLY, TJ
影响因子：
64.5
作者：
DERMAN, E;KRAUTER, K;DARNELL, JE
通讯作者：
DARNELL, JE
DOI：
10.1073/pnas.79.18.5597
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CASTELLOT, JJ;KARNOVSKY, MJ;SPIEGELMAN, BM
通讯作者：
SPIEGELMAN, BM
影响因子：
3.5
作者：
MESSING, J;VIEIRA, J
通讯作者：
VIEIRA, J