Perfusion decellularization of a human limb: A novel platform for composite tissue engineering and reconstructive surgery.
Perfusion decellularization of a human limb: A novel platform for composite tissue engineering and reconstructive surgery.
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人体肢体的灌注脱细胞：复合组织工程和重建手术的新型平台。
DOI：
10.1371/journal.pone.0191497
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Ott HC
中科院分区：
文献类型：
--
作者：
Gerli MFM;Guyette JP;Evangelista-Leite D;Ghoshhajra BB;Ott HC
从自体供体部位获取的肌肉和筋膜皮瓣是目前最常用的创伤修复方法，仅在美国每年就进行了 450 万例手术。然而，供体组织的大小是有限的，并且与这些手术技术相关的并发症会导致发病，通常涉及供体部位。另外，最近的报告表明，细胞外基质（ECM）支架可以增强受伤部位的再生潜力，正如一小群体积性肌肉损失患者所显示的那样。灌注脱细胞是一种生物工程技术，可以从各种供体器官和组织中生成具有保留的复杂结构和完整血管模板的临床规模的 ECM 支架。我们最近报道说，这项技术可以从大鼠和非人类灵长类动物的四肢中生成完整的复合组织支架。将此平台应用于人类四肢可以使软组织和体积肌肉损失患者受益匪浅，提供组织和物种特异性移植物。在这项概念验证研究中，我们展示了从完整的人体上肢成功生成大规模脱细胞复合组织支架。该构建体保留了其形态结构和可灌注血管。组织学和生化验证证实成功去除了核和细胞成分，并强调了天然细胞外基质成分的保存。我们的结果表明灌注脱细胞可用于生产人体复合组织脱细胞支架。凭借其保留的结构和血管模板，这些生物相容性构建体在营养分布、尺寸可扩展性和免疫反应方面比目前植入的基质具有显着优势。
Muscle and fasciocutaneous flaps taken from autologous donor sites are currently the most utilized approach for trauma repair, accounting annually for 4.5 million procedures in the US alone. However, the donor tissue size is limited and the complications related to these surgical techniques lead to morbidities, often involving the donor sites. Alternatively, recent reports indicated that extracellular matrix (ECM) scaffolds boost the regenerative potential of the injured site, as shown in a small cohort of volumetric muscle loss patients. Perfusion decellularization is a bioengineering technology that allows the generation of clinical-scale ECM scaffolds with preserved complex architecture and with an intact vascular template, from a variety of donor organs and tissues. We recently reported that this technology is amenable to generate full composite tissue scaffolds from rat and non-human primate limbs. Translating this platform to human extremities could substantially benefit soft tissue and volumetric muscle loss patients providing tissue- and species-specific grafts. In this proof-of-concept study, we show the successful generation a large-scale, acellular composite tissue scaffold from a full cadaveric human upper extremity. This construct retained its morphological architecture and perfusable vascular conduits. Histological and biochemical validation confirmed the successful removal of nuclear and cellular components, and highlighted the preservation of the native extracellular matrix components. Our results indicate that perfusion decellularization can be applied to produce human composite tissue acellular scaffolds. With its preserved structure and vascular template, these biocompatible constructs, could have significant advantages over the currently implanted matrices by means of nutrient distribution, size-scalability and immunological response.
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影响因子：
8.9
作者：
Gilpin, Sarah Elizabeth;Guyette, Jacques P.;Ott, Harald C.
通讯作者：
Ott, Harald C.
DOI：
10.5435/00124635-201102001-00007
发表时间：
2011-01-01
影响因子：
3.2
作者：
Grogan, Brian F.;Hsu, Joseph R.
通讯作者：
Hsu, Joseph R.
影响因子：
21.3
作者：
Dellavalle, Arianna;Sampaolesi, Maurilio;Cossu, Giulio
通讯作者：
Cossu, Giulio
影响因子：
7.2
作者：
Dziki, Jenna;Badylak, Stephen;Rubin, J. Peter
通讯作者：
Rubin, J. Peter
DOI：
10.1196/annals.1413.004
发表时间：
2008-01-01
期刊：
LYMPHATIC CONTINUUM REVISITED
影响因子：
--
作者：
Hitchcock, Thomas;Niklason, Laura
通讯作者：
Niklason, Laura