Natural blood feeding and temperature shift modulate the global transcriptional profile of Rickettsia rickettsii infecting its tick vector.
Natural blood feeding and temperature shift modulate the global transcriptional profile of Rickettsia rickettsii infecting its tick vector.
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DOI：
10.1371/journal.pone.0077388
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Fogaça AC
中科院分区：
文献类型：
--
作者：
Galletti MF;Fujita A;Nishiyama MY Jr;Malossi CD;Pinter A;Soares JF;Daffre S;Labruna MB;Fogaça AC
立克次体是一种专性细胞内蜱传细菌，引起落基山斑点热（RMSF），最致命的斑点热立克次体病。当受感染的饥饿蜱开始从脊椎动物宿主那里吸血时，R。立克次氏体暴露于温度升高和血粉中的成分。这两种环境刺激以前已经与蜱中立克次体毒力的重新激活有关，但负责这种表型转换的因素尚未完全阐明。使用定制的寡核苷酸微阵列和高通量微流控qRT-PCR，我们分析了10°C温度升高和血餐对R.立克次氏体感染蜱Amblyomma aureolatum。这是第一次研究感染天然蜱载体的立克次氏体属细菌的转录组。虽然这两种刺激都显着增加了细菌负荷，但血液喂养的影响更大，调节的基因是温度升高的五倍。IV型分泌系统（T4 SS）的某些组分通过血液喂养而上调。这表明，这种重要的细菌转运系统可用于在蜱虫载体的血餐期间分泌效应物。吸血也上调了抗氧化酶的表达，这可能与R。立克次氏体以保护自身免受由进食的蜱产生的自由基的有害影响。在这项研究中确定的调制基因，包括那些编码假设的蛋白质，需要进一步的功能分析，并可能有潜力作为疫苗开发的未来目标。
Rickettsia rickettsii is an obligate intracellular tick-borne bacterium that causes Rocky Mountain Spotted Fever (RMSF), the most lethal spotted fever rickettsiosis. When an infected starving tick begins blood feeding from a vertebrate host, R. rickettsii is exposed to a temperature elevation and to components in the blood meal. These two environmental stimuli have been previously associated with the reactivation of rickettsial virulence in ticks, but the factors responsible for this phenotype conversion have not been completely elucidated. Using customized oligonucleotide microarrays and high-throughput microfluidic qRT-PCR, we analyzed the effects of a 10°C temperature elevation and of a blood meal on the transcriptional profile of R. rickettsii infecting the tick Amblyomma aureolatum. This is the first study of the transcriptome of a bacterium in the genus Rickettsia infecting a natural tick vector. Although both stimuli significantly increased bacterial load, blood feeding had a greater effect, modulating five-fold more genes than the temperature upshift. Certain components of the Type IV Secretion System (T4SS) were up-regulated by blood feeding. This suggests that this important bacterial transport system may be utilized to secrete effectors during the tick vector’s blood meal. Blood feeding also up-regulated the expression of antioxidant enzymes, which might correspond to an attempt by R. rickettsii to protect itself against the deleterious effects of free radicals produced by fed ticks. The modulated genes identified in this study, including those encoding hypothetical proteins, require further functional analysis and may have potential as future targets for vaccine development.
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DOI：
10.1073/pnas.41.11.870
发表时间：
1955-01-01
影响因子：
11.1
作者：
GILFORD, JH;PRICE, WH
通讯作者：
PRICE, WH
影响因子：
3.1
作者：
HAYES, SF;BURGDORFER, W
通讯作者：
BURGDORFER, W
影响因子：
3.7
作者：
Gillespie JJ;Ammerman NC;Dreher-Lesnick SM;Rahman MS;Worley MJ;Setubal JC;Sobral BS;Azad AF
通讯作者：
Azad AF
DOI：
10.1111/j.1749-6632.2009.04516.x
发表时间：
2009-01-01
期刊：
RICKETTSIOLOGY AND RICKETTSIAL DISEASES
影响因子：
--
作者：
Labruna, Marcelo B.
通讯作者：
Labruna, Marcelo B.
影响因子：
5.8
作者：
Ballman, KV;Grill, DE;Therneau, TM
通讯作者：
Therneau, TM