Virulence determinants, drug resistance and mobile genetic elements of Laribacter hongkongensis: a genome-wide analysis.
Virulence determinants, drug resistance and mobile genetic elements of Laribacter hongkongensis: a genome-wide analysis.
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DOI：
10.1186/2045-3701-1-17
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发表时间：
2011-04-19
影响因子：
7.5
通讯作者：
Woo PC
中科院分区：
文献类型：
--
作者：
Lau SK;Wong GK;Tsang AK;Teng JL;Fan RY;Tse H;Yuen KY;Woo PC
香港落叶松杆菌与社区获得性胃肠炎和旅行者腹泻有关。在这项研究中，我们对其基因组中与感染过程、耐药性和移动遗传元件各个步骤相关的基因进行了深入注释。对于酸和胆汁抵抗，L. hongkongensis 拥有一个脲酶基因盒、两个 arc 基因簇和胆汁盐流出系统。对于肠道定植，它具有与广泛粘附的大肠杆菌（E. coli）和产肠毒素大肠杆菌同源的自转运蛋白家族的推定粘附素。为了逃避宿主防御，它拥有超氧化物歧化酶和过氧化氢酶。对于脂多糖生物合成，它与大肠杆菌具有相同的一组编码合成脂质A的酶、两个Kdo单位和庚糖单位的基因，但其对称酰化模式的基因不同，以及九个多糖侧链生物合成基因。它包含许多编码假定的细胞表面作用（RTX 毒素和溶血素）和细胞内细胞毒素（patatin 样蛋白）和入侵酶（外膜磷脂酶 A）的 CDS。它包含多种抗生素耐药性相关基因，包括与 β-内酰胺 (n = 10) 和多药外排 (n = 54) 相关的基因。它还包含 8 个前噬菌体、17 个其他噬菌体相关 CDS 和 26 个转座酶 CDS。香港乳杆菌基因组拥有耐酸和胆汁、肠粘膜定植、逃避宿主防御以及细胞毒性和侵袭的基因。还鉴定了多种抗生素抗性或多药抗性基因、大量原噬菌体、其他噬菌体相关的 CDS 和转座酶的 CDS。
Laribacter hongkongensis is associated with community-acquired gastroenteritis and traveler's diarrhea. In this study, we performed an in-depth annotation of the genes in its genome related to the various steps in the infective process, drug resistance and mobile genetic elements. For acid and bile resistance, L. hongkongensis possessed a urease gene cassette, two arc gene clusters and bile salt efflux systems. For intestinal colonization, it possessed a putative adhesin of the autotransporter family homologous to those of diffusely adherent Escherichia coli (E. coli) and enterotoxigenic E. coli. To evade from host defense, it possessed superoxide dismutase and catalases. For lipopolysaccharide biosynthesis, it possessed the same set of genes that encode enzymes for synthesizing lipid A, two Kdo units and heptose units as E. coli, but different genes for its symmetrical acylation pattern, and nine genes for polysaccharide side chains biosynthesis. It contained a number of CDSs that encode putative cell surface acting (RTX toxin and hemolysins) and intracellular cytotoxins (patatin-like proteins) and enzymes for invasion (outer membrane phospholipase A). It contained a broad variety of antibiotic resistance-related genes, including genes related to β-lactam (n = 10) and multidrug efflux (n = 54). It also contained eight prophages, 17 other phage-related CDSs and 26 CDSs for transposases. The L. hongkongensis genome possessed genes for acid and bile resistance, intestinal mucosa colonization, evasion of host defense and cytotoxicity and invasion. A broad variety of antibiotic resistance or multidrug resistance genes, a high number of prophages, other phage-related CDSs and CDSs for transposases, were also identified.
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