First Cryo-Scanning Electron Microscopy Images and X-Ray Microanalyses of Mucoromycotinian Fine Root Endophytes in Vascular Plants.
First Cryo-Scanning Electron Microscopy Images and X-Ray Microanalyses of Mucoromycotinian Fine Root Endophytes in Vascular Plants.
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DOI：
10.3389/fmicb.2020.02018
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发表时间：
2020
影响因子：
5.2
通讯作者：
Ryan MH
中科院分区：
文献类型：
--
作者：
Albornoz FE;Hayes PE;Orchard S;Clode PL;Nazeri NK;Standish RJ;Bending GD;Hilton S;Ryan MH
产生丛枝的细根内生菌（FRE）（以前不正确的Glomus tenue）最近被置于毛霉菌亚门；丛枝菌属亚门外首次报道。在这里，我们的目的是估计FRE植物和真菌结构中的营养浓度，并测试冷冻扫描电子显微镜（cryoSEM）在研究这些真菌方面的实用性。我们对地下三叶草（Trifolium subterranum）的重定植根进行了复制冷冻电镜和x射线显微分析。细胞间菌丝和发达的丛枝菌丝一致很薄；直径分别为1.35±0.03 μm和0.99±0.03 μm（平均值±SE）。几个细胞间菌丝常常彼此相邻，形成“菌丝绳”。发育的丛枝比衰老的丛枝和未定植结构的丛枝显示出更高的磷浓度。衰老的丛枝显示钙和镁的浓度显著升高。虽然均匀纤细的菌丝和菌丝绳是FRE的显著特征，但完全发育的丛枝形态、真菌结构中磷的升高以及衰老丛枝中钙的积累与结构完整性的丧失与肾小球菌系真菌相似。因此，我们提供的证据表明，FRE可能对类似的寄主-植物信号作出反应，或者寄主植物可能采用与FRE和AMF相关的类似机制。
Arbuscule-producing fine root endophytes (FRE) (previously incorrectly Glomus tenue) were recently placed within subphylum Mucoromycotina; the first report of arbuscules outside subphylum Glomeromycotina. Here, we aimed to estimate nutrient concentrations in plant and fungal structures of FRE and to test the utility of cryo-scanning electron microscopy (cryoSEM) for studying these fungi. We used replicated cryoSEM and X-ray microanalysis of heavily colonized roots of Trifolium subterraneum. Intercellular hyphae and hyphae in developed arbuscules were consistently very thin; 1.35 ± 0.03 μm and 0.99 ± 0.03 μm in diameter, respectively (mean ± SE). Several intercellular hyphae were often adjacent to each other forming “hyphal ropes.” Developed arbuscules showed higher phosphorus concentrations than senesced arbuscules and non-colonized structures. Senesced arbuscules showed greatly elevated concentrations of calcium and magnesium. While uniformly thin hyphae and hyphal ropes are distinct features of FRE, the morphology of fully developed arbuscules, elevated phosphorus in fungal structures, and accumulation of calcium with loss of structural integrity in senesced arbuscules are similar to glomeromycotinian fungi. Thus, we provide evidence that FRE may respond to similar host-plant signals or that the host plant may employ a similar mechanism of association with FRE and AMF.
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影响因子：
1.1
作者：
ABBOTT, LK
通讯作者：
ABBOTT, LK
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
2.8
作者：
KINDEN, DA;BROWN, MF
通讯作者：
BROWN, MF
影响因子：
4.6
作者：
Feijen FAA;Vos RA;Nuytinck J;Merckx VSFT
通讯作者：
Merckx VSFT
影响因子：
6.9
作者：
Hayes, Patrick E.;Clode, Peta L.;Lambers, Hans
通讯作者：
Lambers, Hans