The way is the goal: how SecA transports proteins across the cytoplasmic membrane in bacteria.
The way is the goal: how SecA transports proteins across the cytoplasmic membrane in bacteria.
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DOI：
10.1093/femsle/fny093
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发表时间：
2018-06-01
影响因子：
2.1
通讯作者：
Huber D
中科院分区：
文献类型：
--
作者：
Cranford-Smith T;Huber D
在细菌中，大多数可溶性分泌蛋白（以及革兰氏阴性细菌中的外膜蛋白）通过 Sec 机制跨细胞质膜的易位是由必需的 ATP 酶 SecA 介导的。该机制的核心由 SecA 和整合膜蛋白 SecYEG 组成，它们在膜中形成蛋白质传导通道。 Sec 机制通过内部编码的靶向信号来识别蛋白质，该信号通常采用 N 端信号序列的形式。此外，在易位之前，底物蛋白必须在细胞质中保持未折叠构象，以便能够通过 SecYEG 进行易位。底物蛋白的识别是通过 SecA 进行的，可以通过 SecA 直接识别，也可以通过将蛋白质递送至 SecA 的分子伴侣进行二次识别。然后通过 SecYEG 筛选底物蛋白是否存在功能信号序列。具有功能信号序列的蛋白质以 ATP 依赖性方式跨膜转运。此处回顾了当前对每个步骤进行调查的研究。作者回顾了目前关于蛋白质如何在细菌中被识别和传递以进行 SecA 介导的易位的研究。
In bacteria, translocation of most soluble secreted proteins (and outer membrane proteins in Gram-negative bacteria) across the cytoplasmic membrane by the Sec machinery is mediated by the essential ATPase SecA. At its core, this machinery consists of SecA and the integral membrane proteins SecYEG, which form a protein conducting channel in the membrane. Proteins are recognised by the Sec machinery by virtue of an internally encoded targeting signal, which usually takes the form of an N-terminal signal sequence. In addition, substrate proteins must be maintained in an unfolded conformation in the cytoplasm, prior to translocation, in order to be competent for translocation through SecYEG. Recognition of substrate proteins occurs via SecA—either through direct recognition by SecA or through secondary recognition by a molecular chaperone that delivers proteins to SecA. Substrate proteins are then screened for the presence of a functional signal sequence by SecYEG. Proteins with functional signal sequences are translocated across the membrane in an ATP-dependent fashion. The current research investigating each of these steps is reviewed here. The authors review current research into how proteins are recognised and delivered for SecA-mediated translocation in bacteria.
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DOI：
10.1083/jcb.200412019
发表时间：
2005-04-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cannon KS;Or E;Clemons WM Jr;Shibata Y;Rapoport TA
通讯作者：
Rapoport TA
影响因子：
5.6
作者：
Chen, Yu;Bauer, Benedikt W.;Rapoport, Tom A.;Gumbart, James C.
通讯作者：
Gumbart, James C.
影响因子：
4.8
作者：
Baars, L;Ytterberg, AJ;de Gier, JW
通讯作者：
de Gier, JW
影响因子：
64.8
作者：
BASSFORD, P;BECKWITH, J
通讯作者：
BECKWITH, J
影响因子：
2.9
作者：
Auclair, Sarah M.;Moses, Julia P.;Mukerji, Ishita
通讯作者：
Mukerji, Ishita