Human snRNA genes use polyadenylation factors to promote efficient transcription termination.
Human snRNA genes use polyadenylation factors to promote efficient transcription termination.
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DOI：
10.1093/nar/gkt892
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发表时间：
2014-01
影响因子：
14.9
通讯作者：
Murphy S
中科院分区：
文献类型：
--
作者：
O'Reilly D;Kuznetsova OV;Laitem C;Zaborowska J;Dienstbier M;Murphy S
RNA聚合酶II转录蛋白质编码和非编码RNA基因，并且在酵母中，不同的机制终止两种基因类型的转录。mRNA基因的转录终止与切割和多聚腺苷酸化错综复杂地结合，而小核仁（sno）/小核（sn）RNA基因的转录由RNA结合蛋白Nrd 1、Nab 3和Sen 1终止。人类中Nrd 1样通路的存在尚未得到证实。使用U1和U2基因作为模型，我们表明，人类snRNA基因更类似于mRNA基因比酵母snRNA基因的终止。整合子复合物取代mRNA切割和多聚腺苷酸化特异性因子复合物，以促进切割并将snRNA 3′-末端加工与终止偶联。此外，与Pta 1（APT）和切割因子I/II复合物相关的成员作为人类snRNA基因的转录终止子，在snRNA 3′端加工中几乎没有作用。基因特异性因子，近端序列元件结合转录因子（PTF），有助于清除核小体的U1和U2基因，这为pol II提供了一个容易的通道，负延伸因子有助于在核小体水平增加的基因末端终止。因此，人snRNA基因可以使用染色质结构作为促进体内有效转录终止的额外机制。
RNA polymerase II transcribes both protein coding and non-coding RNA genes and, in yeast, different mechanisms terminate transcription of the two gene types. Transcription termination of mRNA genes is intricately coupled to cleavage and polyadenylation, whereas transcription of small nucleolar (sno)/small nuclear (sn)RNA genes is terminated by the RNA-binding proteins Nrd1, Nab3 and Sen1. The existence of an Nrd1-like pathway in humans has not yet been demonstrated. Using the U1 and U2 genes as models, we show that human snRNA genes are more similar to mRNA genes than yeast snRNA genes with respect to termination. The Integrator complex substitutes for the mRNA cleavage and polyadenylation specificity factor complex to promote cleavage and couple snRNA 3′-end processing with termination. Moreover, members of the associated with Pta1 (APT) and cleavage factor I/II complexes function as transcription terminators for human snRNA genes with little, if any, role in snRNA 3′-end processing. The gene-specific factor, proximal sequence element-binding transcription factor (PTF), helps clear the U1 and U2 genes of nucleosomes, which provides an easy passage for pol II, and the negative elongation factor facilitates termination at the end of the genes where nucleosome levels increase. Thus, human snRNA genes may use chromatin structure as an additional mechanism to promote efficient transcription termination in vivo.
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影响因子：
10.5
作者：
Luo, WF;Johnson, AW;Bentley, DL
通讯作者：
Bentley, DL
影响因子：
5.3
作者：
Egloff, Sylvain;Al-Rawaf, Hadeel;Murphy, Shona
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Murphy, Shona
影响因子：
4.8
作者：
Egloff, Sylvain;Szczepaniak, Sylwia Anna;Murphy, Shona
通讯作者：
Murphy, Shona
影响因子：
16
作者：
Buratowski S
通讯作者：
Buratowski S
影响因子：
64.8
作者：
Kim, M;Krogan, NJ;Buratowski, S
通讯作者：
Buratowski, S