A minority of muscarinic receptors mediate rabbit tracheal smooth muscle contraction.
A minority of muscarinic receptors mediate rabbit tracheal smooth muscle contraction.
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少数毒蕈碱受体介导兔气管平滑肌收缩。
DOI：
10.1165/ajrcmb/6.3.279
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发表时间：
1992
影响因子：
6.4
通讯作者：
Bloom,JW
中科院分区：
文献类型：
--
作者：
Mahesh,VK;Nunan,LM;Halonen,M;Yamamura,HI;Palmer,JD;Bloom,JW
材料和方法膜制备新西兰白色兔，雌雄不限，体重1.5 - 2.5 kg，麻醉后断头处死。打开胸腔，取出心脏和肺。将气管和心房小心地切开并立即使用或在-700 ℃冷冻直至使用（1个月内）.从Pel-Freez Biologicals（Rogers，AR）获得处死后立即冷冻的其他新西兰白色家兔气管。在新鲜和冷冻组织之间的解离常数（Kd）值和受体的最大密度（Brnax）中没有观察到结果差异（数据未显示）。在测定时，解剖后肌带（气管平滑肌），上皮完整，丢弃剩余的气管组织。在一些制备中，用3 mm支气管细胞学刷（Mill-Rose Laboratories，Mentor，OH）去除上皮。进行组织学检查以确认上皮细胞的去除。
Materials and MethodsMembrane Preparation New Zealand White rabbits of either sex weighing 1.5 to 2.5 kg were anesthetized and killed by decapitation. The thorax was opened, and the heart and lungs were removed. The trachea and atria were carefully dissected free and used immediately or frozen at-700 C until use (within 1 mo). Additional New Zealand White rabbit trachea, frozen immediately after killing, were obtained from Pel-Freez Biologicals (Rogers, AR). No difference in results was noted in the dissociation constant (Kd) values and the maximum densities of receptors (Brnax) between the fresh and frozen tissue (data not shown).At the time of assay, the posterior muscle band (tracheal smooth muscle) was dissected with the epithelium intact, and the remainder of the tracheal tissue was discarded. In some preparations, the epithelium was removed with a 3-mm bronchial cytology brush (Mill-Rose Laboratories, Mentor, OH). Histologic examination was performed to confirm removal of epithelium.
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激动剂与脑毒蕈碱受体的结合。
影响因子：
3.6
作者：
N. Birdsall;A. Burgen;E. Hulme
通讯作者：
E. Hulme
毒蕈碱胆碱能受体在调节哺乳动物脑、心肌和肠平滑肌中鸟苷 3:5-环单磷酸含量中的作用。
DOI：
--
发表时间：
1972
影响因子：
11.1
作者：
T. P. Lee;J. Kuo;P. Greengard
通讯作者：
P. Greengard
DOI：
--
发表时间：
1987
期刊：
American Review of Respiratory Disease
影响因子：
--
作者：
P. Barnes
通讯作者：
P. Barnes
猪气管平滑肌毒蕈碱胆碱受体的生化特征。
DOI：
--
发表时间：
1986
期刊：
Journal of autonomic pharmacology
影响因子：
--
作者：
C. Yang;J. Farley;T. Dwyer
通讯作者：
T. Dwyer
胆碱能控制气道平滑肌。
DOI：
10.1164/ajrccm/136.4_pt_2.s42
发表时间：
1987
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Peter J. Barnes
通讯作者：
Peter J. Barnes