Antihyperalgesic Effect of Hesperidin Improves with Diosmin in Experimental Neuropathic Pain.
Antihyperalgesic Effect of Hesperidin Improves with Diosmin in Experimental Neuropathic Pain.
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DOI：
10.1155/2016/8263463
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发表时间：
2016
影响因子：
--
通讯作者：
López-Muñoz FJ
中科院分区：
文献类型：
--
作者：
Carballo-Villalobos AI;González-Trujano ME;Pellicer F;López-Muñoz FJ
神经病理性疼痛是由周围或中枢神经系统的原发损害、功能障碍或一过性扰动引起的。在这项研究中，我们在神经病理性疼痛模型中研究了橙皮苷单独或与地奥司明合用的抗痛觉过敏作用，以证实可能的协同抗伤害活性。大鼠慢性缩窄性损伤模型后，分别给予橙皮苷(HS，5个剂量，10~1000 mg/kg)或与地奥司明(DS，10,100 mg/kg)合用，并与加巴喷丁(31.6 mg/kg)比较，分别在感觉计和足底实验中观察痛觉过敏和热过敏反应。通过对脑组织样品的UHPLC-MS分析来识别这些黄酮类化合物的中心浓度。在氟哌啶醇、荷包牡丹碱和纳洛酮拮抗剂存在的情况下，也研究了不同受体的参与。急性给予橙皮苷可显著降低CCI大鼠的机械性和热敏性痛敏。氟哌啶醇、荷包牡丹碱和纳洛酮可阻断橙皮苷的抗过敏反应，但不能阻断WAY100635。橙皮苷与地奥明(DS10/HS100)联合应用可改善这两种刺激的痛敏反应。在大脑样本中都检测到了这两种黄酮类化合物。综上所述，橙皮苷单独及与地欧明合用可在大鼠CCI模型中产生抗痛觉过敏反应。D2、GABAA和阿片类药物部分调节中枢活动，但不受5-HT1a受体调节，从而发挥抗痛敏作用。
Neuropathic pain is caused by a primary lesion, dysfunction, or transitory perturbation in the peripheral or central nervous system. In this study, we investigated the hesperidin antihyperalgesic effects alone or combined with diosmin in a model of neuropathic pain to corroborate a possible synergistic antinociceptive activity. Mechanical and thermal hyperalgesia were assessed in the aesthesiometer and plantar tests, respectively, after chronic constriction injury (CCI) model in rats receiving hesperidin (HS, 5 doses from 10 to 1000 mg/kg) alone or combined with diosmin (DS, 10 and 100 mg/kg) in comparison to gabapentin (31.6 mg/kg). UHPLC-MS analysis of cerebral samples was used to recognize the central concentrations of these flavonoids. Participation of different receptors was also investigated in the presence of haloperidol, bicuculline, and naloxone antagonists. Acute hesperidin administration significantly decreased mechanical and thermal hyperalgesia in CCI rats. Antihyperalgesic response of hesperidin, improved by a combination with diosmin (DS10/HS100) in both stimuli, was blockaded by haloperidol, bicuculline, and naloxone, but not WAY100635, antagonists. Both flavonoids were detected in brain samples. In conclusion, hesperidin alone and combined with diosmin produces antihyperalgesic response in the CCI model in rats. Antihyperalgesic effect of DS10/HS100 combination involves central activity partially modulated by D2, GABAA, and opioids, but not by 5-HT1A, receptors.
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影响因子：
3.6
作者：
Hagenacker, Tim;Hillebrand, Imke;Schaefers, Maria
通讯作者：
Schaefers, Maria
影响因子：
2.7
作者：
Hemsley, KM;Hopwood, JJ
通讯作者：
Hopwood, JJ
影响因子：
7.4
作者：
BENNETT, GJ;XIE, YK
通讯作者：
XIE, YK
影响因子：
3.6
作者：
Laura Martinez, Ana;Eva Gonzalez-Trujano, Ma;Lopez-Munoz, Francisco J.
通讯作者：
Lopez-Munoz, Francisco J.
影响因子：
2.8
作者：
JEAN, T;BODINIER, MC
通讯作者：
BODINIER, MC