Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix.
Microfluidic separation of axonal and somal compartments of neural progenitor cells differentiated in a 3D matrix.
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DOI：
10.1016/j.xpro.2021.101028
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Bhattacharyya R
中科院分区：
文献类型：
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作者：
Lotlikar MS;Tarantino MB;Jorfi M;Kovacs DM;Tanzi RE;Bhattacharyya R
This protocol describes the differentiation of human neural progenitor cells (hNPCs) in a microfluidic device containing a thin 3D matrix with two separate chambers, enabling a cleaner separation between axons and soma/bulk neurons. We have used this technique to study how mitochondria-associated ER membranes (MAMs) regulate the generation of somal and axonal amyloid β (Aβ) in FAD hNPCs, a cellular model of Alzheimer’s disease. This protocol also details the quantification of Aβ molecules and isolation of pure axons via axotomy. For complete details on the use and execution of this profile, please refer to. 3D microfluidic separation of axons from soma of AD neural cellular model, FAD hNPCs Quantitation of axonal versus somal Aβ molecules released from FAD hNPCs Isolation of pure axons from differentiated FAD hNPCs via axotomy Application to show that MAM-modulation regulates axonal Aβ generation in AD This protocol describes the differentiation of human neural progenitor cells (hNPCs) in a microfluidic device containing a thin 3D matrix with two separate chambers, enabling a cleaner separation between axons and soma/bulk neurons. We have used this technique to study how mitochondria-associated ER membranes (MAMs) regulate the generation of somal and axonal amyloid β (Aβ) in FAD hNPCs, a cellular model of Alzheimer’s disease. This protocol also details the quantification of Aβ molecules and isolation of pure axons via axotomy.
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影响因子：
16.6
作者：
Kwak, Sang Su;Washicosky, Kevin J.;Kim, Doo Yeon
通讯作者：
Kim, Doo Yeon
DOI：
10.1073/pnas.1000645107
发表时间：
2010-04-13
影响因子：
11.1
作者：
Takeda, Shuko;Sato, Naoyuki;Morishita, Ryuichi
通讯作者：
Morishita, Ryuichi
影响因子：
8.8
作者：
Bhattacharyya R;Black SE;Lotlikar MS;Fenn RH;Jorfi M;Kovacs DM;Tanzi RE
通讯作者：
Tanzi RE
影响因子：
25
作者：
Das U;Wang L;Ganguly A;Saikia JM;Wagner SL;Koo EH;Roy S
通讯作者：
Roy S
影响因子：
14.8
作者：
通讯作者：
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