Distinct membrane fractions from mouse sperm bind different zona pellucida glycoproteins.
Distinct membrane fractions from mouse sperm bind different zona pellucida glycoproteins.
复制标题
小鼠精子的不同膜部分结合不同的透明带糖蛋白。
DOI：
10.1095/biolreprod66.1.65
复制
发表时间：
2002
影响因子：
3.6
通讯作者：
Cardullo,RichardA
中科院分区：
文献类型：
--
作者：
Thaler,CatherineD;Cardullo,RichardA
Interactions between sperm and zona pellucida (ZP) during mammalian fertilization are not well characterized at the molecular level. To identify sperm proteins that recognize ligand ZP3, we used sonicated sperm membrane fractions as competitors in a quantitative binding assay. Sonicated membranes were density fractionated into 4 fractions. Bands 1–3 contained membrane vesicles, and band 4 contained axonemal and midpiece fragments. In competitive binding assays, bands 1, 2, and 3 but not band 4 were able to compete with live, capacitated, intact sperm for soluble125I-ZP binding. Affinity-purified ZP fractions consisting of a ZP3-enriched fraction (125I-ZP3) and a fraction enriched for ligands ZP1 and ZP2 and depleted of ZP3 (125I-ZP1/2) were obtained by antibody affinity purification of ZP3. In competitive binding assays, bands 2 and 3 competed for125I-ZP3 binding, but band 1 did not interact with enriched125I-ZP3. None of the membrane fractions competed for125I-ZP1/2 binding. These results demonstrate that band 2 and band 3 contain sperm components that interact with ZP3 alone and that components in band 1 interact with ZP3 in conjunction with either ZP1 or ZP2. These data indicate that there must be at least 2 unique sperm plasma membrane components that mediate intact sperm interactions with ZP glycoproteins in mouse. Bands 2 and 3 are likely to contain a primary ZP-binding protein because they interacted directly with ZP3, whereas band 1 may contain sperm proteins involved in later interactions with the ZP, perhaps transitional interactions to maintain sperm contact with the ZP during acrosomal exocytosis.
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编码透明带糖蛋白 ZP3（小鼠精子受体）的基因的核苷酸序列。
影响因子：
14.9
作者：
R. Kinloch;P. M. Wassarman
通讯作者：
P. M. Wassarman
编码 ZP3（小鼠透明带精子结合蛋白）的 cDNA 的分子分析。
影响因子：
2.7
作者：
M. Ringuette;M. Chamberlin;A. Baur;D. Sobieski;J. Dean
通讯作者：
J. Dean
影响因子：
--
作者：
N. Cross
通讯作者：
N. Cross
哺乳动物精子受体基因的概况。
DOI：
--
发表时间：
1989
期刊：
The New biologist
影响因子：
--
作者：
R. A. Kinloch;P. M. Wassarman
通讯作者：
P. M. Wassarman
小鼠卵子发生过程中精子受体的生物合成。
DOI：
10.1002/j.1460-2075.1983.tb01607.x
发表时间：
1983
期刊：
The EMBO Journal
影响因子：
--
作者：
G. S. Salzmann;J. Greve;R. Roller;P. M. Wassarman
通讯作者：
P. M. Wassarman