Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration.
Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration.
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DOI：
10.3892/etm.2017.4637
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发表时间：
2017-08
影响因子：
2.7
通讯作者：
He F
中科院分区：
文献类型：
--
作者：
Lin H;Zhang W;Zhou T;Li W;Chen Z;Ji C;Zhang C;He F
本研究探讨了microRNA-21在椎间盘退变中调节IL-6炎症反应和细胞自噬的机制。选择10例腰椎间盘突出伴神经根痛患者（观察组）和10例腰椎爆裂骨折患者（对照组）。术中取病变髓核组织进行细胞培养。采用实时定量聚合酶链反应（PCR）检测microRNA-21的表达。ELISA法检测IL-6、ⅱ型胶原蛋白（Col II）水平。采用聚集蛋白和western blotting检测自噬相关基因7 （ATG7）和微管相关蛋白1轻链3 (LC3)-II/−I。结果观察组患者的microRNA-21、IL-6水平显著高于对照组，Col II、aggrecan水平显著低于对照组。差异有统计学意义（P<0.05）。观察组患者ATG7、LC3-II/−I水平均显著降低（P<0.05）。综上所述，microRNA-21在腰椎间盘神经根痛中表达异常高，可增加IL-6炎症反应，降低细胞自噬能力。
This study investigated the mechanism of microRNA-21 in regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration. A total of 10 patients with lumbar disc herniation accompanied by nerve root pain (observation group) and 10 patients with lumbar burst fractures (control group) were selected. The nucleus pulposus tissues of the lesion were obtained during operation for cell culture. Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of microRNA-21. The ELISA method was used to detect the levels of IL-6, and type II collagen (Col II). Aggrecan and western blotting was used to detect autophagy-related gene 7 (ATG7) and microtubule-associated protein 1 light chain 3 (LC3)-II/−I. As a result, the levels of microRNA-21 and IL-6 in the observation group were significantly higher than those in the control group, but the levels of Col II and aggrecan were significantly lower than those in the control group. The differences were statistically significant (P<0.05). The levels of ATG7 and LC3-II/−I in the observation group were significantly decreased (P<0.05). In conclusion, the expression of microRNA-21 is abnormally high in the nerve root pain of the lumbar intervertebral disc, which can increase the IL-6 inflammatory response and reduce the capacity of cell autophagy.
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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影响因子：
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作者：
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影响因子：
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作者：
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