T-tubule disease: Relationship between t-tubule organization and regional contractile performance in human dilated cardiomyopathy.
T-tubule disease: Relationship between t-tubule organization and regional contractile performance in human dilated cardiomyopathy.
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DOI：
10.1016/j.yjmcc.2015.04.022
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发表时间：
2015-07
影响因子：
5
通讯作者：
Cannell, Mark B.
中科院分区：
文献类型：
--
作者：
Crossman, David J.;Young, Alistair A.;Ruygrok, Peter N.;Nason, Guy P.;Baddelely, David;Soeller, Christian;Cannell, Mark B.
来自动物模型的证据表明，t-小管的变化可能在与心力衰竭相关的收缩缺陷中起重要作用。然而，样本通常是随机采集的，不考虑衰竭心脏中存在的区域变异性，这导致收缩性能和可能的t-小管紊乱之间的关系不确定。用标记电影MRI和模型拟合测量了人心脏的局部收缩。在移植时，在确定的区域对衰竭的心脏进行活检取样，并使用免疫细胞化学标记t-小管和肌节z-线。计算机图像分析用于评估5种不同的t-小管结构/组织的无偏测量。在表现出良好收缩性能的衰竭心脏区域，t-小管组织与正常心脏相似，结构恶化与区域收缩性能丧失相关。统计分析表明，t-小管方向是最高度相关的局部收缩性能，其次是肌节峰的幅度在傅立叶变换的t-小管图像。其他基于区域的措施相关性较差。我们的结论是，区域收缩性能在失败的人类心脏是密切相关的，与当地的t-小管组织。聚类树分析与功能定义的失败收缩强度允许的病理定义的“t-小管疾病”。收缩性能和细胞结构的区域变异性是从衰竭的人类心脏中采集的样本分析的混杂问题，尽管这可以通过使用标记的cMRI和活检映射进行区域分析来克服。
Evidence from animal models suggest that t-tubule changes may play an important role in the contractile deficit associated with heart failure. However samples are usually taken at random with no regard as to regional variability present in failing hearts which leads to uncertainty in the relationship between contractile performance and possible t-tubule derangement. Regional contraction in human hearts was measured by tagged cine MRI and model fitting. At transplant, failing hearts were biopsy sampled in identified regions and immunocytochemistry was used to label t-tubules and sarcomeric z-lines. Computer image analysis was used to assess 5 different unbiased measures of t-tubule structure/organization. In regions of failing hearts that showed good contractile performance, t-tubule organization was similar to that seen in normal hearts, with worsening structure correlating with the loss of regional contractile performance. Statistical analysis showed that t-tubule direction was most highly correlated with local contractile performance, followed by the amplitude of the sarcomeric peak in the Fourier transform of the t-tubule image. Other area based measures were less well correlated. We conclude that regional contractile performance in failing human hearts is strongly correlated with the local t-tubule organization. Cluster tree analysis with a functional definition of failing contraction strength allowed a pathological definition of ‘t-tubule disease’. The regional variability in contractile performance and cellular structure is a confounding issue for analysis of samples taken from failing human hearts, although this may be overcome with regional analysis by using tagged cMRI and biopsy mapping.
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影响因子：
3.7
作者：
Crossman DJ;Ruygrok PN;Soeller C;Cannell MB
通讯作者：
Cannell MB
影响因子：
19.7
作者：
Moore, CC;Lugo-Olivieri, CH;Zerhouni, EA
通讯作者：
Zerhouni, EA
DOI：
10.1198/106186006x133933
发表时间：
2006-09-01
影响因子：
2.4
作者：
Hothorn, Torsten;Hornik, Kurt;Zeileis, Achim
通讯作者：
Zeileis, Achim
影响因子：
2.7
作者：
Cannell, M. B.;Crossman, D. J.;Soeller, C.
通讯作者：
Soeller, C.
影响因子：
37.8
作者：
SCHAPER, J;FROEDE, R;BLEESE, N
通讯作者：
BLEESE, N