Synthesis and Characterization of Elastin-Mimetic Hybrid Polymers with Multiblock, Alternating Molecular Architecture and Elastomeric Properties.
Synthesis and Characterization of Elastin-Mimetic Hybrid Polymers with Multiblock, Alternating Molecular Architecture and Elastomeric Properties.
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DOI：
10.1021/ma802791z
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发表时间：
2009-04
期刊：
影响因子：
5.5
通讯作者：
Jia, Xinqiao
中科院分区：
文献类型：
--
作者：
Grieshaber, Sarah E.;Farran, Alexandra J. E.;Lin-Gibson, Sheng;Kiick, Kristi L.;Jia, Xinqiao
我们有兴趣开发弹性蛋白模拟杂交聚合物（EMHPs），捕获原弹性蛋白的多嵌段分子结构以及成熟弹性蛋白的显着弹性。在这项研究中，多嵌段EMHPs含有灵活的合成片段的基础上，聚（乙二醇）（PEG）交替与富含丙氨酸，含赖氨酸的肽使用α，ω-叠氮基-PEG和炔封端的AKA 3 KA（K =赖氨酸，A =丙氨酸）肽，采用正交点击化学逐步增长聚合。所得EMHP含有估计的三至五个重复的PEG和AKA 3 KA，并具有34 kDa的平均分子量。虽然肽单独在高pH下表现出α-螺旋结构，但EMHP的螺旋度分数降低。EMHP通过肽结构域中的赖氨酸残基与二异氰酸酯（HMDI）共价交联，得到水合时压缩模量为0.12 MPa的弹性体水凝胶（xEMHP）。在相同条件下，xEMHP的机械性能与市售聚氨酯弹性体（Tecoflex SG 80 A）相当。体外毒性研究表明，虽然可溶性EMHP在浓度≥0.2 mg/mL时可抑制原代猪声带成纤维细胞（PVFF）的生长，但交联混合弹性体不会浸出任何毒性试剂，并允许PVFF正常生长和增殖。混合和模块化的方法提供了一种新的策略，为开发弹性支架的组织工程。
We are interested in developing elastin–mimetic hybrid polymers (EMHPs) that capture the multiblock molecular architecture of tropoelastin as well as the remarkable elasticity of mature elastin. In this study, multiblock EMHPs containing flexible synthetic segments based on poly(ethylene glycol) (PEG) alternating with alanine-rich, lysine-containing peptides were synthesized by step-growth polymerization using α,ω-azido-PEG and alkyne-terminated AKA3KA (K = lysine, A = alanine) peptide, employing orthogonal click chemistry. The resulting EMHPs contain an estimated three to five repeats of PEG and AKA3KA and have an average molecular weight of 34 kDa. While the peptide alone exhibited α-helical structures at high pH, the fractional helicity for EMHPs was reduced. Covalent cross-linking of EMHPs with hexamethylene diisocyanate (HMDI) through the lysine residue in the peptide domain afforded an elastomeric hydrogel (xEMHP) with a compressive modulus of 0.12 MPa when hydrated. The mechanical properties of xEMHP are comparable to a commercial polyurethane elastomer (Tecoflex SG80A) under the same conditions. In vitro toxicity studies showed that while the soluble EMHPs inhibited the growth of primary porcine vocal fold fibroblasts (PVFFs) at concentrations ≥0.2 mg/mL, the cross-linked hybrid elastomers did not leach out any toxic reagents and allowed PVFFs to grow and proliferate normally. The hybrid and modular approach provides a new strategy for developing elastomeric scaffolds for tissue engineering.
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影响因子：
3.3
作者：
Gent, AN
通讯作者：
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影响因子：
2.9
作者：
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作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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