The synthesis of yeast pyruvate decarboxylase is regulated by large variations in the messenger RNA level

The synthesis of yeast pyruvate decarboxylase is regulated by large variations in the messenger RNA level
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酵母丙酮酸脱羧酶的合成受到信使 RNA 水平的巨大变化的调节

DOI:
10.1007/bf00327674
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发表时间:
2004
期刊:
Molecular and General Genetics MGG
影响因子:
--
通讯作者:
F. Zimmermann
F. Zimmermann
中科院分区:
--
文献类型:
--
作者:
H. Schmitt;M. Ciriacy;F. Zimmermann

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摘要分离到了酵母菌丙酮酸脱羧酶编码基因PDC1。该DNA序列用于杂交鉴定相应的信使RNA。由此可见,丙酮酸脱羧酶的合成受PDC1 mRNA含量变化的有效调控。在含有乙醇的培养基中生长的细胞中发现PDC1 mRNA水平非常低。葡萄糖添加到这些细胞导致PDC1 mRNA的快速积累。在不同的突变体和在含有葡萄糖或乙醇以外的碳源培养基中生长的细胞中发现的PDC1 mRNA水平表明,酵母细胞中PDC1 mRNA的数量受到许多不同因素的影响。
SummaryThe yeast PDC1 gene coding for the fermentative enzyme pyruvate decarboxylase was isolated. This DNA sequence was used to identify the corresponding messenger RNA by hybridization. It could be shown that the synthesis of pyruvate decarboxylase is efficiently regulated by variations in the amount of PDC1 mRNA. Very low levels of PDC1 mRNA were found in cells growing in a medium containing ethanol. Glucose addition to these cells leads to a rapid accumulation of PDC1 mRNA. The PDC1 mRNA levels found in different mutants and in cells growing in media containing carbon sources other than glucose or ethanol suggest that the amount of PDC1 mRNA in yeast cells is affected by a number of different factors.
微生物糖酵解的生化遗传学。
DOI: 10.1007/978-1-4684-3980-9_13
发表时间: 1981
期刊: Basic life sciences
影响因子: --
作者:
Fraenkel,DG
通讯作者: Fraenkel,DG
DOI: 10.1016/s0021-9258(18)33174-0
发表时间: 1983-01
期刊: The Journal of biological chemistry
影响因子: --
作者:
C. Denis;J. Ferguson;E. Young
通讯作者: C. Denis;J. Ferguson;E. Young
酵母磷酸果糖激酶的突变研究。
DOI: 10.1021/bi00537a037
发表时间: 1982
期刊: Biochemistry
影响因子: 2.9
作者:
Clifton,D;Fraenkel,DG
通讯作者: Fraenkel,DG
酿酒酵母的 gcr(糖酵解调节)突变。
DOI: --
发表时间: 1981
期刊: The Journal of biological chemistry
影响因子: --
作者:
Clifton,D;Fraenkel,DG
通讯作者: Fraenkel,DG
DOI: --
发表时间: 1982
期刊: The Journal of biological chemistry
影响因子: --
作者:
McAlister,L;Holland,MJ
通讯作者: Holland,MJ