Dosimetry of irradiation models. The 96-well clonogenic assay for testing radiosensitivity of cell lines.
Dosimetry of irradiation models. The 96-well clonogenic assay for testing radiosensitivity of cell lines.
复制标题
辐射模型的剂量测定。
作者：
J. Kulmala;V. Rantanen;K. Pekkola‐Heino;J. Tuominen;R. Grénman
对细胞在试管和96孔板上的单细胞悬液进行了辐射实验，并进行了比较。这些细胞来源于口底癌和子宫内膜癌的细胞系。建立了两个辐照模型。两种模型都允许在两种实验设置下以高精度（优于5.0%）给予细胞吸收剂量。这些辐照模型在具有不同固有辐射敏感性和组织学类型（UM-SCC-1耐药，UM-SCC-14A敏感，UT-EC-2B高度敏感）的癌细胞系上进行比较；使用了不同的辐射剂量。比较了存活细胞的比例与辐射剂量的关系：试管辐照细胞与96孔板辐照细胞无显著差异。因此，如果悬浮在管中的细胞和悬浮在板中的细胞的吸收剂量相同，则无论辐照模型的类型如何，存活都是相似的。
Radiation experiments with cells in single cell suspension in test tubes and on 96-well plates were carried out and compared. The cells originated from cell lines established from carcinomas of the floor of the mouth and from endometrial carcinoma. Two irradiation models were constructed. Both models allowed the absorbed doses to the cells to be administered with a high accuracy in both experimental settings (better than 5.0%). These irradiation models were compared on cancer cell lines with dissimilar inherent radiation sensitivity and histologic type (UM-SCC-1 resistant, UM-SCC-14A sensitive, and UT-EC-2B highly sensitive); various radiation doses were used. The fractions of surviving cells as a function of radiation dose were compared: there was no significant difference between cells irradiated in test tubes and cells irradiated in 96-well plates. Thus, if the absorbed doses in cells suspended in a tube and in a plate were the same, the survival was similar regardless of the type of irradiation model.
影响因子：
--
作者：
C. Krause;T. Carey;R. Ott;Charles G. Hurbis;K. Mcclatchey;J. Regezi
通讯作者：
C. Krause;T. Carey;R. Ott;Charles G. Hurbis;K. Mcclatchey;J. Regezi
头颈癌细胞的体外放射敏感性。
DOI：
10.1001/archotol.1988.01860160071024
发表时间：
1988
期刊：
Archives of otolaryngology--head & neck surgery
影响因子：
--
作者：
Grenman,R;Burk,D;Virolainen,E;Wagner,JG;Lichter,AS;Carey,TE
通讯作者：
Carey,TE
外阴鳞状细胞癌（UM-SCV-1A、UM-SCV-1B、UM-SCV-2 和 A-431）细胞的体外放射反应。
影响因子：
11.2
作者：
Pekkola-Heino,K;Kulmala,J;Grenman,S;Carey,TE;Grenman,R
通讯作者：
Grenman,R