Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis.
Circulating microRNAs in Symptomatic and Asymptomatic Carotid Stenosis.
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DOI：
10.3389/fneur.2021.755827
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发表时间：
2021
影响因子：
3.4
通讯作者：
Weissenborn K
中科院分区：
文献类型：
--
作者：
Grosse GM;Derda AA;Stauss RD;Neubert L;Jonigk DD;Kühnel MP;Gabriel MM;Schuppner R;Wilhelmi M;Bär C;Bauersachs J;Schrimpf C;Thum T;Weissenborn K
背景资料：特异性microRNAs（miRs）参与了动脉粥样硬化的病理生理过程，可能成为颈动脉狭窄的诊断和治疗靶点。我们假设，与计划接受颈动脉内膜切除术（CEA）的无症状颈动脉狭窄（aCS）患者相比，有症状颈动脉狭窄（sCS）患者的特异性循环miR水平发生了变化。我们还研究了miR水平是否与CEA后随时间推移的斑块脆弱性和稳定性相关。研究方法：在CEA前和90天随访时，在21例sCS患者和23例aCS患者中测定了血管富集的miR-92 a、miR-126、miR-143、miR-145、miR-155、miR-210、miR-221、miR-222和miR-342- 3 p的循环水平。在CEA之前进行经颅多普勒超声检测同侧大脑中动脉中的微栓子信号（MES）。对颈动脉斑块进行组织学分析。结果如下：miR-92 a、miR-210、miR-145和miR-143的平均水平在组间无显著差异，sCS中仅略高于aCS，其中miR-92 a的证据最充分。在调整血管危险因素和他汀类药物预处理后，效应量基本保持不变。然而，在随访时，这些微小的差异仍未得到证实。miR水平与MES或组织学斑块易损性特征之间无相关性。结论：这项研究没有提供证据表明，在一组综合特征的颈动脉狭窄患者中，特定循环miR与症状状态之间存在强相关性。需要进一步的工作来阐明循环miR作为晚期颈动脉粥样硬化靶点的作用。
Background: Specific microRNAs (miRs) have been implicated in the pathophysiology of atherosclerosis and may represent interesting diagnostic and therapeutic targets in carotid stenosis. We hypothesized that the levels of specific circulating miRs are altered in patients with symptomatic carotid stenosis (sCS) in comparison to those in patients with asymptomatic carotid stenosis (aCS) planned to undergo carotid endarterectomy (CEA). We also studied whether miR levels are associated with plaque vulnerability and stability over time after CEA. Methods: Circulating levels of vascular-enriched miR-92a, miR-126, miR-143, miR-145, miR-155, miR-210, miR-221, miR-222, and miR-342-3p were determined in 21 patients with sCS and 23 patients with aCS before CEA and at a 90-day follow-up. Transcranial Doppler ultrasound for detection of microembolic signals (MES) in the ipsilateral middle cerebral artery was performed prior to CEA. Carotid plaques were histologically analyzed. Results: Mean levels of miRs were not considerably different between groups and were only marginally higher in sCS than aCS concerning miR-92a, miR-210, miR-145, and miR-143 with the best evidence concerning miR-92a. After adjustment for vascular risk factors and statin pre-treatment, the effect sizes remained essentially unchanged. At follow-up, however, these modest differences remained uncorroborated. There were no relevant associations between miR-levels and MES or histological plaque vulnerability features. Conclusions: This study does not provide evidence for strong associations between specific circulating miRs and symptomatic state in a collective of comprehensively characterized patients with carotid stenosis. Further work is needed to elucidate the role of circulating miRs as targets in advanced carotid atherosclerosis.
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影响因子：
5.2
作者：
Pordzik J;Pisarz K;De Rosa S;Jones AD;Eyileten C;Indolfi C;Malek L;Postula M
通讯作者：
Postula M
影响因子：
64.8
作者：
Cordes KR;Sheehy NT;White MP;Berry EC;Morton SU;Muth AN;Lee TH;Miano JM;Ivey KN;Srivastava D
通讯作者：
Srivastava D
影响因子：
20.1
作者：
Loyer, Xavier;Potteaux, Stephane;Tedgui, Alain
通讯作者：
Tedgui, Alain
影响因子：
20.1
作者：
Eken, Suzanne M.;Jin, Hong;Maegdefessel, Lars
通讯作者：
Maegdefessel, Lars
影响因子：
20.1
作者：
Ridker, Paul M.
通讯作者：
Ridker, Paul M.