Validation of a murine proteome-wide phage display library for identification of autoantibody specificities.
Validation of a murine proteome-wide phage display library for identification of autoantibody specificities.
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DOI：
10.1172/jci.insight.174976
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发表时间：
2023-12-08
期刊：
影响因子：
8
通讯作者：
Derisi, Joseph L.
中科院分区：
文献类型：
--
作者：
Rackaityte, Elze;Proekt, Irina;Miller, Haleigh S.;Ramesh, Akshaya;Brooks, Jeremy F.;Kung, Andrew F.;Mandel-Brehm, Caleigh;Yu, David;Zamecnik, Colin R.;Bair, Rebecca;Vazquez, Sara E.;Sunshine, Sara;Abram, Clare L.;Lowell, Clifford A.;Rizzuto, Gabrielle;Wilson, Michael R.;Zikherman, Julie;Anderson, Mark S.;Derisi, Joseph L.
自身免疫的特征在于对组织特异性以及全身性抗原的耐受性丧失，导致复杂的自身抗体景观。在这里，我们介绍并广泛验证了噬菌体展示免疫沉淀和测序（PhIP-seq）在分析小鼠自身抗体的鼠蛋白质组全文库的性能特征。该文库使用7种遗传上不同的小鼠品系在一系列自身反应性中进行验证。使用抗体产生缺陷的小鼠（Rag 2-/-和μMT）模拟非特异性肽富集，同时使用抗卵清蛋白B细胞受体限制性OB 1小鼠评价交叉反应性作为原理证明。然后利用PhIP-seq方法来询问3种不同的自身免疫性疾病模型。首先，来自患有狼疮样疾病的林恩-/- IgD+/-小鼠的血清用于鉴定核和凋亡滤过泡反应性。第二，非肥胖糖尿病（NOD）小鼠，胰腺特异性自身免疫的多基因模型，血清中富含胰岛素和预测的胰腺蛋白衍生的肽。最后，Aire-/-小鼠血清被用于鉴定许多自身抗原，其中许多也在先前的研究中观察到的人类自身免疫性多内分泌病综合征1型携带隐性突变的AIRE。这些实验支持使用鼠蛋白质组范围的PhIP-seq进行抗原分析和自身抗体发现，其可用于研究自身免疫分析的小鼠模型中的一系列免疫扰动。
Autoimmunity is characterized by loss of tolerance to tissue-specific as well as systemic antigens, resulting in complex autoantibody landscapes. Here, we introduce and extensively validate the performance characteristics of a murine proteome-wide library for phage display immunoprecipitation and sequencing (PhIP-seq) in profiling mouse autoantibodies. This library was validated using 7 genetically distinct mouse lines across a spectrum of autoreactivity. Mice deficient in antibody production (Rag2–/– and μMT) were used to model nonspecific peptide enrichments, while cross-reactivity was evaluated using anti-ovalbumin B cell receptor–restricted OB1 mice as a proof of principle. The PhIP-seq approach was then utilized to interrogate 3 distinct autoimmune disease models. First, serum from Lyn–/– IgD+/– mice with lupus-like disease was used to identify nuclear and apoptotic bleb reactivities. Second, serum from nonobese diabetic (NOD) mice, a polygenic model of pancreas-specific autoimmunity, was enriched in peptides derived from both insulin and predicted pancreatic proteins. Lastly, Aire–/– mouse sera were used to identify numerous autoantigens, many of which were also observed in previous studies of humans with autoimmune polyendocrinopathy syndrome type 1 carrying recessive mutations in AIRE. These experiments support the use of murine proteome-wide PhIP-seq for antigenic profiling and autoantibody discovery, which may be employed to study a range of immune perturbations in mouse models of autoimmunity profiling.
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DOI：
10.1093/bioinformatics/btr064
发表时间：
2011-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Grant CE;Bailey TL;Noble WS
通讯作者：
Noble WS
影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
影响因子：
56.9
作者：
Anderson, MS;Venanzi, ES;Mathis, D
通讯作者：
Mathis, D
DOI：
10.1084/jem.194.8.1151
发表时间：
2001-10-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hao Z;Rajewsky K
通讯作者：
Rajewsky K
自身免疫性多边形综合征1型的鼠模型中自身免疫综合征的效应器机制。
影响因子：
4.4
作者：
DeVoss, Jason J.;Shum, Anthony K.;Johannes, Kellsey P. A.;Lu, Wen;Krawisz, Anna K.;Wang, Peter;Yang, Ting;LeClair, Norbert P.;Austin, Cecilia;Strauss, Erich C.;Anderson, Mark S.
通讯作者：
Anderson, Mark S.