The microbiome extends to subepidermal compartments of normal skin.
The microbiome extends to subepidermal compartments of normal skin.
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DOI：
10.1038/ncomms2441
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发表时间：
2013
影响因子：
16.6
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中科院分区：
文献类型：
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皮肤表面的共生微生物影响表皮下细胞的行为。我们假设细菌或其产物存在于表皮下，因此允许微生物和真皮细胞之间的物理相互作用。为了验证这一假设，我们采用了多种独立的细菌检测技术，包括qPCR、革兰氏染色、免疫荧光和原位杂交。在正常人皮肤的真皮和真皮脂肪内始终可检测到细菌。真皮和真皮脂肪组织的DNA测序鉴定了细菌16S rRNA，反映了每个皮肤隔室中的多样性和部分不同的微生物群落。这些结果表明，微生物群在真皮内延伸，因此使细菌和基底膜下的各种细胞之间能够物理接触。这些观察结果表明，正常的肠道细菌群落直接与宿主在以前被认为是无菌的组织中交流。
Commensal microbes on the skin surface influence the behavior of cells below the epidermis. We hypothesized that bacteria or their products exist below the surface epithelium and thus permit physical interaction between microbes and dermal cells. Here, to test this hypothesis, we employed multiple independent detection techniques for bacteria including qPCR, Gram-staining, immunofluorescence, and in situ hybridization. Bacteria were consistently detectable within the dermis and dermal adipose of normal human skin. Sequencing of DNA from dermis and dermal adipose tissue identified bacterial 16S rRNA reflective of a diverse and partially distinct microbial community in each skin compartment. These results show the microbiota extends within the dermis, therefore enabling physical contact between bacteria and various cells below the basement membrane. These observations show that normal commensal bacterial communities directly communicate with the host in a tissue previously thought to be sterile.
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影响因子：
3.7
作者：
Dowd SE;Wolcott RD;Sun Y;McKeehan T;Smith E;Rhoads D
通讯作者：
Rhoads D
影响因子：
3.7
作者：
Hirama T;Yamaguchi T;Miyazawa H;Tanaka T;Hashikita G;Kishi E;Tachi Y;Takahashi S;Kodama K;Egashira H;Yokote A;Kobayashi K;Nagata M;Ishii T;Nemoto M;Tanaka M;Fukunaga K;Morita S;Kanazawa M;Hagiwara K
通讯作者：
Hagiwara K
影响因子：
6.5
作者：
Capone, Kimberly A.;Dowd, Scot E.;Stamatas, Georgios N.;Nikolovski, Janeta
通讯作者：
Nikolovski, Janeta
影响因子：
56.9
作者：
Kravchenko, Vladimir V.;Kaufmann, Gunnar F.;Ulevitch, Richard J.
通讯作者：
Ulevitch, Richard J.
DOI：
10.1038/jid.2009.384
发表时间：
2010-04
期刊：
The Journal of investigative dermatology
影响因子：
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