Immunohistochemical Studies of Insulin-Like Growth Factor-I in Corpora Lutea of Cycling and Pregnant Mice, Rats, Hamsters, Guinea Pigs and Cattle

Immunohistochemical Studies of Insulin-Like Growth Factor-I in Corpora Lutea of Cycling and Pregnant Mice, Rats, Hamsters, Guinea Pigs and Cattle
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骑自行车和怀孕小鼠、大鼠、仓鼠、豚鼠和牛黄体中胰岛素样生长因子-I 的免疫组织化学研究

DOI:
10.1262/jrd.40.79
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发表时间:
1994
影响因子:
1.8
通讯作者:
J. Mori
J. Mori
中科院分区:
生物学3区
文献类型:
--
作者:
H. Tamada;M. Ohno;T. Sawada;J. Mori

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比较了小鼠、大鼠、仓鼠、豚鼠和黄牛发情周期和妊娠黄体中胰岛素样生长因子-I(IGF-I)的免疫组织化学染色。在发情周期中,金黄地鼠的CL未见明显的染色,而小鼠、大鼠和豚鼠的CL除排卵后1天的CL染色很弱外,其余均呈弱染色。而牛CL中的IGF-I免疫反应有明显的变化。虽然在卵泡和黄体早期有非常微弱的染色,但之后染色强度显著增加,在黄体晚期达到峰值。在妊娠过程中,小鼠和大鼠CL均呈阳性染色,妊娠晚期染色强度增强,尤以大鼠CL为强染色。在仓鼠CL中,仅在妊娠晚期才检测到明显的阳性染色。相反,在豚鼠和牛CL中,虽然在妊娠中期有强烈的染色,但在妊娠晚期染色减少。结果表明,在CL中免疫活性IGF-I的积累存在物种差异。
Immunohistochemical staining of insulin-like growth factor-I (IGF-I) in the corpora lutea (CL) during estrous cycle and pregnancy was compared among mice, rats, hamsters, guinea pigs and cattle. During the estrous cycle, while clear staining was not detected in the hamster CL, the CL of the mouse, rat and guinea pig showed modest staining except for the mouse CL 1 day after ovulation which showed very weak staining. In contrast, immunoreactive IGF-I in the bovine CL showed distinct changes. While very weak staining was noted during the follicular and early luteal phases, the intensity increased markedly thereafter, peaking at the late luteal phase. During preg-nancy, the mouse and rat CL showed positive staining, and the intensity increased at late preg-nancy, especially in the rat CL intense focal staining was noted. In the hamster CL, clear positive staining was detected only at late pregnancy. In contrast, in the guinea-pig and bovine CL, al-though intense staining was detected during mid-pregnancy, the staining decreased at late preg-nancy. The results revealed species differences in the accumulation of immunoreactive IGF-I in CL.
大鼠黄体细胞对胰岛素样生长因子 I (IGF-I) 反应的研究:黄素化大鼠卵巢中 IGF-I 特异性细胞膜受体的鉴定。
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