Specific binding of endothelin on human vascular smooth muscle cells in culture.
Specific binding of endothelin on human vascular smooth muscle cells in culture.
复制标题
内皮素对培养的人血管平滑肌细胞的特异性结合。
DOI：
10.1172/jci114078
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
C. Guilly
中科院分区：
文献类型：
--
作者：
Martine Clozel;W. Fischli;C. Guilly
内皮素是一种新发现的由内皮细胞分泌的强缩血管肽。本文研究了内皮素与培养的人脐静脉血管平滑肌细胞的结合。确定了125 I-内皮素的单一特异性结合位点，表观Kd为126 pM，最大结合容量为每个平滑肌细胞约10，000个位点。在室温下的结合是饱和的，在2小时达到平衡（使用20 pM内皮素），并缓慢地，只有部分逆转未标记的内皮素。钙拮抗剂硝苯地平、尼卡地平和地尔硫卓不竞争相同的结合位点。培养的人脐静脉内皮细胞条件培养液呈剂量依赖性地抑制~（125）I-内皮素结合。这种作用被抗内皮素抗血清所拮抗。我们的结论是，人脐静脉平滑肌细胞具有内皮素的特异性结合位点，人内皮细胞分泌内皮素样物质。
Endothelin is a newly discovered, potent vasoconstrictor peptide secreted by endothelial cells. The binding of endothelin was studied on cultured human vascular smooth muscle cells obtained from umbilical veins. A single specific binding site for 125I-endothelin was identified, with an apparent Kd of 126 pM and a maximal binding capacity of approximately 10,000 sites per smooth muscle cell. At room temperature the binding was saturable, reached equilibrium at 2 h (using 20 pM endothelin), and was slowly and only partially reversed by unlabeled endothelin. The calcium antagonists nifedipine, nicardipine, and diltiazem did not compete for the same binding site. Conditioned medium from cultured human umbilical vein endothelial cells inhibited the binding of 125I-endothelin dose dependently. This effect was antagonized by anti-endothelin antiserum. We conclude that human umbilical vein smooth muscle cells possess specific binding sites for endothelin, and that human endothelial cells secrete an endothelinlike material.
DOI：
--
发表时间：
1975-07
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
M. Gimbrone;R. Cotran
通讯作者：
M. Gimbrone;R. Cotran