Intranuclear arrangement of human chromosome 12 correlates to large-scale replication domains
Intranuclear arrangement of human chromosome 12 correlates to large-scale replication domains
复制标题
人类 12 号染色体的核内排列与大规模复制域相关
作者：
M. Nogami;Orie Nogami;K. Kagotani;M. Okumura;H. Taguchi;T. Ikemura;K. Okumura
摘要。采用多色荧光原位杂交（FISH）技术，以带特异性cosmid克隆为探针，分析了人髓性白血病HL60细胞G0（G1）核内12号染色体的排列。在多聚甲醛固定的HL60细胞核上检测到不同颜色的小粒子对，并对其在单个细胞核内或外周的相对位置进行评分。我们的结果表明，人类12号染色体的核内排列不是随机的。在G0（G1）细胞中，一些染色体结构域，包括着丝粒，位于细胞核的外围，而其他结构域，包括端粒，位于细胞核的内部区域。根据中期扩散的复制带带模式，将人类12号染色体大致划分为5个大结构域。有趣的是，后期复制区域的克隆优先定位于细胞核外围，而早期复制区域的克隆则分布在细胞核内部。每个cosmid的DNA复制时间并不反映复制带，但复制时间的总体概况与12号染色体上的这些结构域相对相关。这些结果表明，人类染色体的核内排列与大规模复制结构域相关，甚至在DNA复制之前。
Abstract.The intranuclear arrangement of human chromosome 12 in G0(G1) nuclei from human myeloid leukemia HL60 cells was analyzed by multicolor fluorescence in situ hybridization (FISH) using band-specific cosmid clones as probes. Pairs of differently colored cosmids were detected on paraformaldehyde-fixed HL60 nuclei, and their relative positions, internal or peripheral, in individual nuclei were scored. Our results suggest that the intranuclear arrangement of human chromosome 12 is not random. Some chromosomal domains, including the centromere, were located in the periphery of the nucleus, while other domains, including the telomeres, were positioned in the internal areas of the nucleus in G0(G1) cells. Based on the replication banding patterns of metaphase spreads, human chromosome 12 was divided roughly into five large domains. Interestingly, the clones in late replicating domains were preferentially localized in the nuclear periphery, whereas clones in early replicating domains were arranged in the internal areas of the nuclei. The DNA replication timing of each cosmid determined by FISH-based assay did not reflect the replication bands, but an overall profile of the replication timing was relatively correlated with these domains on chromosome 12. These results suggest that the intranuclear arrangement of a human chromosome is correlated with the large-scale replication domains, even before DNA replication.
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12 号染色体特异的人类 α 卫星 DNA 子集。
影响因子：
9.8
作者：
Baldini,A;Rocchi,M;Archidiacono,N;Miller,OJ;Miller,DA
通讯作者：
Miller,DA
影响因子：
11.4
作者：
SELIG, S;OKUMURA, K;CEDAR, H
通讯作者：
CEDAR, H
DOI：
10.1073/pnas.85.23.9138
发表时间：
1988-12-01
影响因子：
11.1
作者：
PINKEL, D;LANDEGENT, J;GRAY, J
通讯作者：
GRAY, J
DOI：
10.1073/pnas.91.13.6083
发表时间：
1994-06-21
影响因子：
11.1
作者：
BOGGS, BA;CHINAULT, C
通讯作者：
CHINAULT, C