Pyrite-induced hydroxyl radical formation and its effect on nucleic acids.
Pyrite-induced hydroxyl radical formation and its effect on nucleic acids.
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DOI：
10.1186/1467-4866-7-3
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发表时间：
2006-04-04
影响因子：
2.3
通讯作者：
Schoonen MA
中科院分区：
文献类型：
--
作者：
Cohn CA;Mueller S;Wimmer E;Leifer N;Greenbaum S;Strongin DR;Schoonen MA
黄铁矿是地球上最丰富的金属硫化物，已知当暴露于水时会自发形成过氧化氢。在这项研究中，假设黄铁矿诱导的过氧化氢转化为羟基自由基进行了测试。使用电子自旋共振（ESR）自旋捕获技术和清除反应，涉及核酸的组合，形成的羟基自由基在黄铁矿/水悬浮液的证明。添加EDTA的黄铁矿浆料抑制过氧化氢到羟基自由基的转化，但不抑制过氧化氢的形成。考虑到EDTA与亚铁和三价铁螯合的稳定性，这表明EDTA的加入防止了溶解的铁物质通过螯合的转化。虽然过氧化氢到羟基自由基转化的确切机制或机制不能根据本研究中报道的实验来解决，但很明显，黄铁矿表面促进了反应。羟基自由基的形成是重要的，因为它们几乎立即与大多数有机分子反应。这表明，黄铁矿在自然，工程或生理水系统中的存在下，可能会引起广泛的有机分子的转化。这一发现对黄铁矿在水生环境中可能发挥的作用产生了影响，并提出了吸入黄铁矿粉尘是否有助于肺部疾病发展的问题。
Pyrite, the most abundant metal sulphide on Earth, is known to spontaneously form hydrogen peroxide when exposed to water. In this study the hypothesis that pyrite-induced hydrogen peroxide is transformed to hydroxyl radicals is tested. Using a combination of electron spin resonance (ESR) spin-trapping techniques and scavenging reactions involving nucleic acids, the formation of hydroxyl radicals in pyrite/aqueous suspensions is demonstrated. The addition of EDTA to pyrite slurries inhibits the hydrogen peroxide-to-hydroxyl radical conversion, but does not inhibit the formation of hydrogen peroxide. Given the stability of EDTA chelation with both ferrous and ferric iron, this suggests that the addition of the EDTA prevents the transformation by chelation of dissolved iron species. While the exact mechanism or mechanisms of the hydrogen peroxide-to-hydroxyl radical conversion cannot be resolved on the basis of the experiments reported in this study, it is clear that the pyrite surface promotes the reaction. The formation of hydroxyl radicals is significant because they react nearly instantaneously with most organic molecules. This suggests that the presence of pyrite in natural, engineered, or physiological aqueous systems may induce the transformation of a wide range of organic molecules. This finding has implications for the role pyrite may play in aquatic environments and raises the question whether inhalation of pyrite dust contributes to the development of lung diseases.
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影响因子：
3.1
作者：
Guevremont, JM;Strongin, DR;Schoonen, MAA
通讯作者：
Schoonen, MAA
影响因子：
6.6
作者：
BIEGLER, T;SWIFT, DA
通讯作者：
SWIFT, DA
影响因子：
11.4
作者：
Cohn, CA;Laffers, R;Schoonen, MAA
通讯作者：
Schoonen, MAA
影响因子：
2.3
作者：
Cohn CA;Pak A;Strongin D;Schoonen MA
通讯作者：
Schoonen MA
影响因子：
4.7
作者：
BERGER, M;DEHAZEN, M;CADET, J
通讯作者：
CADET, J