Electrophoretic collision of a DNA molecule with an insulating post.

Electrophoretic collision of a DNA molecule with an insulating post.
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DNA 分子与绝缘柱的电泳碰撞。

DOI:
10.1103/physrevlett.93.058102
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发表时间:
2004
影响因子:
8.6
通讯作者:
P. Doyle
P. Doyle
中科院分区:
物理与天体物理1区
文献类型:
--
作者:
G. Randall;P. Doyle

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我们研究了单个DNA分子在电场驱动下进入静止障碍物的动力学。这些碰撞大致分为“挂钩”事件和“滚落”事件。我们发现,障碍物诱导的电场梯度拉伸影响DNA,从而极大地影响钩概率。因此,除了碰撞几何外,挂钩概率的确定还取决于0.5<De<40时的黛博拉数(De)。个体DNA碰撞动力学具有高度的构型敏感性,具有聚合物在长形流和场中的特点。
We study the dynamics of single DNA molecules driven by an electric field into a stationary obstacle. These collisions are broadly classified as "hook" and "roll-off" events. We show that obstacle-induced electric field gradients stretch impacting DNA and thus greatly influence the hooking probability. Consequently, in addition to collision geometry, determination of the hooking probability depends on the Deborah number (De) for 0.5<De<40. Individual DNA impact dynamics are highly configuration sensitive, characteristic of polymers in elongational flows and fields.
DOI: 10.1126/science.276.5321.2016
发表时间: 1997-06-27
期刊: SCIENCE
影响因子: 56.9
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影响因子: 3.4
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