Río-Hortega's drawings revisited with fluorescent protein defines a cytoplasm-filled channel system of CNS myelin.
Río-Hortega's drawings revisited with fluorescent protein defines a cytoplasm-filled channel system of CNS myelin.
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DOI：
10.1111/joa.13577
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发表时间：
2021-12
影响因子：
2.4
通讯作者：
Boullerne A
中科院分区：
文献类型：
--
作者：
Edgar JM;McGowan E;Chapple KJ;Möbius W;Lemgruber L;Insall RH;Nave KA;Boullerne A
一个世纪前的这一年，Pío del Río‐Hortega（1921）为“束间胶质细胞”创造了“少突胶质细胞”一词，对他的新细胞类型进行了广泛的发现。一百年后，我们回顾了他对我们理解髓鞘内细胞质通道系统的原始贡献，在我们今天使用遗传编码荧光报告和免疫染色的光学和电子显微镜观察的背景下。我们使用术语髓鞘通道系统来描述与髓鞘相关的细胞质分隔空间；包括副神经节袢、内、外舌、通过致密髓鞘充满细胞质的空间以及与鞘相关的更复杂的基序。使用包含所有主要神经细胞类型并产生致密髓磷脂的中枢神经系统髓鞘细胞培养模型，我们发现td -番茄荧光蛋白描绘髓鞘通道系统的方式让人想起Río‐Hortega绘制的成人白质，尽管他质疑他观察到的一些细胞质图形是否代表人工制品。总之，这些数据使我们提出了一个稍微修改的“展开”鞘的模型。此外，我们表明髓鞘通道系统虽然相对稳定，但可以在数天内经历微妙的动态形状变化。重要的是，我们捕获了成熟髓鞘中髓鞘通道系统的复杂性。利用荧光报告蛋白和免疫染色，我们重新审视Pío del Río‐Hortega对中枢神经系统髓鞘细胞质的精美描绘。总之，这些数据突出了髓鞘通道系统未被充分认识的复杂性。我们采用三维重建来说明一些关键特征。
A century ago this year, Pío del Río‐Hortega (1921) coined the term ‘oligodendroglia’ for the ‘interfascicular glia’ with very few processes, launching an extensive discovery effort on his new cell type. One hundred years later, we review his original contributions to our understanding of the system of cytoplasmic channels within myelin in the context of what we observe today using light and electron microscopy of genetically encoded fluorescent reporters and immunostaining. We use the term myelinic channel system to describe the cytoplasm‐delimited spaces associated with myelin; being the paranodal loops, inner and outer tongues, cytoplasm‐filled spaces through compact myelin and further complex motifs associated to the sheath. Using a central nervous system myelinating cell culture model that contains all major neural cell types and produces compact myelin, we find that td‐tomato fluorescent protein delineates the myelinic channel system in a manner reminiscent of the drawings of adult white matter by Río‐Hortega, despite that he questioned whether some cytoplasmic figures he observed represented artefact. Together, these data lead us to propose a slightly revised model of the ‘unrolled’ sheath. Further, we show that the myelinic channel system, while relatively stable, can undergo subtle dynamic shape changes over days. Importantly, we capture an under‐appreciated complexity of the myelinic channel system in mature myelin sheaths. Using fluorescent reporter protein and immunostaining we revisit Pío del Río‐Hortega’s exquisite drawings of the cytoplasm of CNS myelin. Together, these data highlight an under‐appreciated complexity of the myelinic channel system. We employ 3D reconstructions to illustrate some key features.
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影响因子：
4.7
作者：
Gould, RM;Freund, CM;Feinstein, DL
通讯作者：
Feinstein, DL
DOI：
10.1073/pnas.1811013115
发表时间：
2018-11-13
影响因子：
11.1
作者：
Dutta DJ;Woo DH;Lee PR;Pajevic S;Bukalo O;Huffman WC;Wake H;Basser PJ;SheikhBahaei S;Lazarevic V;Smith JC;Fields RD
通讯作者：
Fields RD
影响因子：
--
作者：
Devaux, Jerome;Fykkolodziej, Bozena;Gow, Alexander
通讯作者：
Gow, Alexander
影响因子：
64.8
作者：
Fuenfschilling, Ursula;Supplie, Lotti M.;Mahad, Don;Boretius, Susann;Saab, Aiman S.;Edgar, Julia;Brinkmann, Bastian G.;Kassmann, Celia M.;Tzvetanova, Iva D.;Moebius, Wiebke;Diaz, Francisca;Meijer, Dies;Suter, Ueli;Hamprecht, Bernd;Sereda, Michael W.;Moraes, Carlos T.;Frahm, Jens;Goebbels, Sandra;Nave, Klaus-Armin
通讯作者：
Nave, Klaus-Armin
DOI：
10.1016/j.cub.2015.07.056
发表时间：
2015-09-21
期刊：
Current biology : CB
影响因子：
--
作者：
Bechler ME;Byrne L;Ffrench-Constant C
通讯作者：
Ffrench-Constant C