In vitro bioactive and immunoactive inhibin concentrations in bovine fetal ovaries and testes throughout gestation.
In vitro bioactive and immunoactive inhibin concentrations in bovine fetal ovaries and testes throughout gestation.
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整个妊娠期间牛胎儿卵巢和睾丸的体外生物活性和免疫活性抑制素浓度。
作者：
A. Torney;D. Robertson;Y. Hodgson;D. D. de Kretser
本研究通过抑制素体外生物测定和RIA技术研究了抑制素在整个妊娠期（40 ~ 270天/足月）牛胎卵巢和睾丸中的浓度。此外，通过Northern blot分析和原位杂交检测抑制素α和β亚基（β A和β B）在这些组织中的表达。在90 d至足月期间，睾丸抑制素浓度的生物活性和免疫活性分别增加2.5倍（103 +/- 24.5 ~ 256 +/- 11.7 U/g湿重；平均+/- SE）和10.4倍（139 +/- 56 ~ 1430 +/- 172）。相应的抑制素生物免疫活性比值（B/I比值）下降8.3倍（1.5 +/- 0.7 ~ 0.18 +/- 0.01）。卵巢生物活性抑制素浓度在妊娠第90天显著增加（P < 0.05） 4.6倍（73.6 +/- 14.7 ~ 340 +/- 11.1 U/g湿重），免疫活性抑制素浓度在妊娠第120 ~ 210天显著增加10.3倍（7.2 +/- 1.9 ~ 40.0 +/- 8.7）。相应的B/I比值在整个妊娠期保持不变（8.3 +/- 2.4 ~ 12.5 +/- 4.0）。尽管在妊娠期间睾丸和卵巢α亚基mRNA水平升高，但睾丸β A亚基mRNA水平保持低水平且不变。卵巢β A亚基mRNA水平也很低，但有变化。此外，在整个妊娠期性腺组织中均未检测到β B亚基mRNA。原位杂交法在胎睾丸性索和胎卵巢颗粒细胞中检测到α亚基mRNA，而β A亚基mRNA仅在胎卵巢颗粒细胞中检测到。由此可见，抑制素是由胎儿睾丸和卵巢产生的，并且在整个妊娠期间这些组织的水平都在增加。α -和β - a亚基mRNA位于睾丸性索和卵巢颗粒细胞，表明这些细胞是抑制素产生的主要来源。在妊娠期睾丸提取物中抑制素B/I比值的快速下降归因于抑制素相关蛋白的产生，其生物活性有限或可以忽略不计。
This study investigates the concentrations of inhibin in the bovine fetal ovary and testis throughout gestation (days 40 to 270/term) as determined by inhibin in vitro bioassay and RIA techniques. In addition, the expression of the inhibin alpha- and beta-subunits (beta A and beta B) in these tissues was evaluated by Northern blot analysis and in situ hybridization. Testicular concentrations of inhibin bioactivity and immunoactivity increased 2.5-fold (103 +/- 24.5 to 256 +/- 11.7 U/g wet weight; mean +/- SE) and 10.4-fold (139 +/- 56 to 1430 +/- 172) respectively, between 90 days and term. The corresponding ratio of inhibin biological: immunological activities (B/I ratio) decreased 8.3-fold (1.5 +/- 0.7 to 0.18 +/- 0.01). The concentration of ovarian bioactive inhibin increased significantly (P less than 0.05) 4.6-fold between 90 days and term (73.6 +/- 14.7 to 340 +/- 11.1 U/g wet weight), whereas the immunoactive inhibin concentration increased 10.3-fold between 120 and 210 days of gestation (7.2 +/- 1.9 to 40.0 +/- 8.7). The corresponding B/I ratio remained unchanged throughout gestation (8.3 +/- 2.4 to 12.5 +/- 4.0). Although the levels of alpha subunit mRNA in the testis and ovary increased over gestation, the levels of testicular beta A subunit mRNA remained low and unchanged. Ovarian levels of beta A subunit mRNA were also low but variable. Furthermore, no beta B subunit mRNA could be detected in gonadal tissue throughout gestation. alpha-Subunit mRNA was detected by in situ hybridization in the sex cords of the fetal testis and the granulosa cells of the fetal ovary while beta A subunit mRNA was detected only in the granulosa cells of the fetal ovary. It is concluded that inhibin is produced by the fetal testis and ovary and these tissue levels increase throughout gestation. The location of alpha- and beta A-subunit mRNA to the sex cords of the testis and granulosa cells of the ovary indicate that these cells are the primary source of inhibin production. The rapid fall in inhibin B/I ratio in testicular extracts over gestation is attributed to the production of an inhibin-related protein with limited or negligible biological activity.
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DOI：
10.1073/pnas.84.23.8282
发表时间：
1987-12
影响因子：
11.1
作者：
Naoto Ueno;N. Ling;Shao-Yao Ying;F. Esch;Shunichi Shimasaki;R. Guillemin
通讯作者：
Naoto Ueno;N. Ling;Shao-Yao Ying;F. Esch;Shunichi Shimasaki;R. Guillemin
Peritz F 检验：稳健多重比较检验的基本程序，用于对组平均值之间的所有差异进行统计分析。
影响因子：
7.7
作者：
Harper,JF
通讯作者：
Harper,JF
大鼠睾丸细胞类型中抑制素亚基的差异产生和调节。
影响因子：
4.8
作者：
Roberts,V;Meunier,H;Sawchenko,PE;Vale,W
通讯作者：
Vale,W
生殖周期期间大鼠卵泡中抑制素信使 RNA 的动态变化。
DOI：
10.1126/science.3125611
发表时间：
1988
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Woodruff,TK;D'Agostino,J;Schwartz,NB;Mayo,KE
通讯作者：
Mayo,KE