Head-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control.
Head-to-tail polymerization by VEL proteins underpins cold-induced Polycomb silencing in flowering control.
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DOI：
10.1016/j.celrep.2022.111607
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发表时间：
2022-11-08
期刊：
影响因子：
8.8
通讯作者：
Bienz M
中科院分区：
文献类型：
--
作者：
Fiedler M;Franco-Echevarría E;Schulten A;Nielsen M;Rutherford TJ;Yeates A;Ahsan B;Dean C;Bienz M
通过Polycomb沉默机制的转录沉默利用涉及组蛋白尾部修饰的“读写”机制。然而，沉默的成核和沉默状态的长期稳定传递也需要P-多梳抑制复合物2（PRC 2）辅助蛋白，其分子作用知之甚少。拟南芥VEL蛋白是辅助蛋白，其与PRC 2相互作用以在FLC基因座处成核和传播沉默，从而使得能够在春季提早开花。在这里，我们报告说，VEL蛋白含有一个域相关的一个非典型的四螺旋束，从事自发的浓度依赖性的头到尾聚合，组装动态生物分子凝聚物。阻断该VEL结构域的聚合的突变防止FLC处的Polycomb沉默。因此，植物VEL蛋白促进沉默状态遗传所需的动态多价多梳复合物的组装。VEL蛋白通过头-尾聚合组装生物分子缩合物，它们的聚合折叠是非典型的四螺旋束，也在辅分子伴侣中发现。报道了VEL蛋白通过头-尾聚合组装生物分子缩合物以促进春天的早花。它们的聚合结构域类似于非典型的四螺旋束，类似于在通用辅助分子伴侣中发现的那些。VEL聚合有助于Polycomb机制持续沉默开花控制基因座。
Transcriptional silencing through the Polycomb silencing machinery utilizes a “read-write” mechanism involving histone tail modifications. However, nucleation of silencing and long-term stable transmission of the silenced state also requires P-olycomb Repressive Complex 2 (PRC2) accessory proteins, whose molecular role is poorly understood. The Arabidopsis VEL proteins are accessory proteins that interact with PRC2 to nucleate and propagate silencing at the FLOWERING LOCUS C (FLC) locus, enabling early flowering in spring. Here, we report that VEL proteins contain a domain related to an atypical four-helix bundle that engages in spontaneous concentration-dependent head-to-tail polymerization to assemble dynamic biomolecular condensates. Mutations blocking polymerization of this VEL domain prevent Polycomb silencing at FLC. Plant VEL proteins thus facilitate assembly of dynamic multivalent Polycomb complexes required for inheritance of the silenced state. VEL proteins assemble biomolecular condensates by head-to-tail polymerization Their polymerizing fold is an atypical four-helix bundle also found in co-chaperones Polymerization of VEL proteins assists transcriptional silencing by Polycomb VEL polymerization is required for the cold-induced transition to flowering Fiedler et al. report that VEL proteins assemble biomolecular condensates by head-to-tail polymerization to promote early flowering in spring. Their polymerizing domain resembles an atypical four-helical bundle akin to those found in universal co-chaperones. VEL polymerization assists the Polycomb machinery in the sustained silencing of a flowering control locus.
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影响因子：
4.4
作者：
Huang, Yong;Jiang, Ling;Ruan, Ying
通讯作者：
Ruan, Ying
影响因子：
13.8
作者：
Craig EA;Marszalek J
通讯作者：
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影响因子：
5.1
作者：
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通讯作者：
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DOI：
10.1073/pnas.79.7.2360
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
INGOLIA, TD;CRAIG, EA
通讯作者：
CRAIG, EA
影响因子：
11.8
作者：
Gambetta, Maria Cristina;Mueller, Juerg
通讯作者：
Mueller, Juerg