Annexin A2 promotes phagophore assembly by enhancing Atg16L⁺ vesicle biogenesis and homotypic fusion.
Annexin A2 promotes phagophore assembly by enhancing Atg16L⁺ vesicle biogenesis and homotypic fusion.
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DOI：
10.1038/ncomms6856
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发表时间：
2015-01-19
影响因子：
16.6
通讯作者：
Santambrogio, Laura
中科院分区：
文献类型：
--
作者：
Morozova, Kateryna;Sidhar, Sunandini;Zolla, Valerio;Clement, Cristina C.;Scharf, Brian;Verzani, Zoe;Diaz, Antonio;Larocca, Jorge N.;Hajjar, Katherine A.;Cuervo, Ana Maria;Santambrogio, Laura
Plasma membrane budding of Atg-16L-positive vesicles represents a very early event in the generation of the phagophore and in the process of macroautophagy. Here we show that the membrane curvature-inducing protein annexin A2 contributes to the formation of these vesicles and their fusion to form phagophores. Ultrastructural, proteomic and FACS analyses of Atg16L-positive vesicles reveal that 30% of Atg16L-positive vesicles are also annexin A2-positive. Lipidomic analysis of annexin A2-deficient mouse cells indicates that this protein plays a role in recruiting phosphatidylserine and phosphatidylinositides to Atg16L-positive vesicles. Absence of annexin A2 reduces both vesicle formation and homotypic Atg16L vesicle fusion. Ultimately, a reduction in LC3 flux and dampening of macroautophagy are observed in dendritic cells from Anxa2−/− mice. Together, our analyses highlight the importance of annexin A2 in vesiculation of a population of Atg16L-positive structures from the plasma membrane, and in their homotypic fusion to form phagophore structures. The earliest steps in autophagy are thought to include the budding of Atg16L-containing vesicles from the plasma membrane and their homotypic fusion to form a phagophore. Morozova et al. reveal a role for the membrane curvature-inducing protein Annexin A2 in the formation and fusion of these vesicles.
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DOI：
10.1083/jcb.201304188
发表时间：
2013-10-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fujita N;Morita E;Itoh T;Tanaka A;Nakaoka M;Osada Y;Umemoto T;Saitoh T;Nakatogawa H;Kobayashi S;Haraguchi T;Guan JL;Iwai K;Tokunaga F;Saito K;Ishibashi K;Akira S;Fukuda M;Noda T;Yoshimori T
通讯作者：
Yoshimori T
影响因子：
11.4
作者：
Hayes, Matthew J.;Shao, Dongmin;Moss, Stephen E.
通讯作者：
Moss, Stephen E.
影响因子：
12.3
作者：
Moss SE;Morgan RO
通讯作者：
Morgan RO
影响因子：
3.3
作者：
Kaushik, Susmita;Massey, Ashish C.;Cuervo, Ana Maria
通讯作者：
Cuervo, Ana Maria
影响因子：
--
作者：
Hedhli N;Falcone DJ;Huang B;Cesarman-Maus G;Kraemer R;Zhai H;Tsirka SE;Santambrogio L;Hajjar KA
通讯作者：
Hajjar KA