Systemic Translocation of Salmonella enterica Serovar Dublin in Cattle Occurs Predominantly via Efferent Lymphatics in a Cell-Free Niche and Requires Type III Secretion System 1 (T3SS-1) but Not T3SS-2
Systemic Translocation of Salmonella enterica Serovar Dublin in Cattle Occurs Predominantly via Efferent Lymphatics in a Cell-Free Niche and Requires Type III Secretion System 1 (T3SS-1) but Not T3SS-2
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牛肠道沙门氏菌都柏林血清型的全身易位主要通过无细胞生态位中的传出淋巴管发生，需要 III 型分泌系统 1 (T3SS-1)，但不需要 T3SS-2
DOI：
10.1128/iai.00784-07
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发表时间：
2007
影响因子：
3.1
通讯作者：
M. Stevens
中科院分区：
文献类型：
--
作者：
G. Pullinger;S. Paulin;B. Charleston;P. Watson;A. Bowen;F. Dziva;E. Morgan;B. Villarreal;T. Wallis;M. Stevens
肠沙门氏菌是一种重要的腹泻病原体，其感染可能涉及严重的全身后遗症，这取决于血清和宿主特异性因素。宿主限制性和特异性肠链球菌血清型从肠道向远端器官易位的分子机制尚不明确。通过对牛回肠远端引流的淋巴管和血管进行手术插管，观察到都柏林肠沙门氏菌主要通过肠系膜淋巴结转移到输出淋巴管，这种方式与系统毒性相关，因为家禽伤寒相关的鸡血清沙门氏菌的易位水平明显较低。虽然都柏林肠链球菌血清型和加利纳姆肠链球菌血清型在肠粘膜中都是细胞内的，并且与主要的组织相容性复合体ii类阳性细胞相关，但在传出淋巴中细菌主要是细胞外的。小牛口服接种后，对标记血清型都柏林突变体文库进行筛选，确定了36个毒力相关位点在肠道和全身感染阶段的作用。口腔感染后早期到达肝脏和脾脏的标记克隆数量和比例与传出淋巴中的值相同，这可能是一种相关的传播方式。合并感染研究证实，淋巴易位需要III型分泌系统1 （T3SS-1）的功能，但值得注意的是，不需要T3SS-2。这是对都柏林血清型在其自然宿主中系统性易位的模式和遗传学的首次描述。
ABSTRACT Salmonella enterica is an important diarrheal pathogen, and infections may involve severe systemic sequelae depending on serovar- and host-specific factors. The molecular mechanisms underlying translocation of host-restricted and -specific serovars of S. enterica from the intestines to distal organs are ill defined. By surgical cannulation of lymph and blood vessels draining the distal ileum in cattle, S. enterica serovar Dublin was observed to translocate predominantly via mesenteric lymph nodes to efferent lymphatics in a manner that correlates with systemic virulence, since the fowl typhoid-associated serovar Gallinarum translocated at a significantly lower level. While both S. enterica serovars Dublin and Gallinarum were intracellular while in the intestinal mucosa and associated with major histocompatibility complex class II-positive cells, the bacteria were predominantly extracellular within efferent lymph. Screening of a library of signature-tagged serovar Dublin mutants following oral inoculation of calves defined the role of 36 virulence-associated loci in enteric and systemic phases of infection. The number and proportion of tagged clones reaching the liver and spleen early after oral infection were identical to the values in efferent lymph, implying that this may be a relevant mode of dissemination. Coinfection studies confirmed that lymphatic translocation requires the function of type III secretion system 1 (T3SS-1) but, remarkably, not T3SS-2. This is the first description of the mode and genetics of systemic translocation of serovar Dublin in its natural host.
影响因子：
2.8
作者：
Carnell, Sonya C.;Bowen, Alison;Stevens, Mark P.
通讯作者：
Stevens, Mark P.
DOI：
10.1073/pnas.86.16.6383
发表时间：
1989-08-01
影响因子：
11.1
作者：
GALAN, JE;CURTISS, R
通讯作者：
CURTISS, R