Novel organisation and regulation of the pic promoter from enteroaggregative and uropathogenic Escherichia coli.
Novel organisation and regulation of the pic promoter from enteroaggregative and uropathogenic Escherichia coli.
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DOI：
10.1080/21505594.2022.2111754
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发表时间：
2022-12
期刊：
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
肠杆菌科丝氨酸蛋白酶自转运蛋白（SPATEs）是肠道细菌中常见的一个毒力因子大家族。这些分泌的毒力因子在细菌感染过程中具有多种功能，包括粘附、聚集和细胞毒性。一种这样的SPATE，Pic粘蛋白酶（参与定殖的蛋白质）切割粘蛋白，允许肠道细菌细胞利用粘蛋白作为碳源并穿透肠道粘液衬里，从而增加粘膜定殖。pic基因广泛分布于肠杆菌科，存在于人类致病菌中，如肠聚集性大肠杆菌（EAEC）、尿路致病性大肠杆菌（EAEC）、大肠杆菌（EAEC）和大肠杆菌（EAEC）。大肠杆菌（UPEC）和福氏志贺菌2a。由于来自EAEC菌株042和UPEC菌株CFT073的pic启动子区域不同，我们研究了每个启动子的调控。在这里，使用体内和体外技术，我们表明，这两个启动子激活的全球转录因子，CRP（环AMP受体蛋白），但EAEC和UPEC PIC启动子的架构不同。两个pic启动子的表达被类核因子Fis抑制，当细胞在基本培养基中生长时，启动子活性最大。由于CRP在营养耗尽的条件下激活转录，而Fis水平在营养丰富的环境中最大，因此EAEC和UPEC pic启动子的调节与Pic在定植和感染期间清除粘蛋白作为合适碳源的营养作用一致。
The serine protease autotransporters of the Enterobacteriaceae (SPATEs) are a large family of virulence factors commonly found in enteric bacteria. These secreted virulence factors have diverse functions during bacterial infection, including adhesion, aggregation and cell toxicity. One such SPATE, the Pic mucinase (protein involved in colonisation) cleaves mucin, allowing enteric bacterial cells to utilise mucin as a carbon source and to penetrate the gut mucus lining, thereby increasing mucosal colonisation. The pic gene is widely distributed within the Enterobacteriaceae, being found in human pathogens, such as enteroaggregative Escherichia coli (EAEC), uropathogenic E. coli (UPEC) and Shigella flexneri 2a. As the pic promoter regions from EAEC strain 042 and UPEC strain CFT073 differ, we have investigated the regulation of each promoter. Here, using in vivo and in vitro techniques, we show that both promoters are activated by the global transcription factor, CRP (cyclic AMP receptor protein), but the architectures of the EAEC and the UPEC pic promoter differ. Expression from both pic promoters is repressed by the nucleoid-associated factor, Fis, and maximal promoter activity occurs when cells are grown in minimal medium. As CRP activates transcription in conditions of nutrient depletion, whilst Fis levels are maximal in nutrient-rich environments, the regulation of the EAEC and UPEC pic promoters is consistent with Pic’s nutritional role in scavenging mucin as a suitable carbon source during colonisation and infection.
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3.2
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