Tissue spheroid fusion-based in vitro screening assays for analysis of tissue maturation.
Tissue spheroid fusion-based in vitro screening assays for analysis of tissue maturation.
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DOI：
10.1002/term.291
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发表时间：
2010-12
影响因子：
3.3
通讯作者：
Visconti, Richard P.
中科院分区：
文献类型：
--
作者：
Hajdu, Zoltan;Mironov, Vladimir;Mehesz, Agnes Nagy;Norris, Russell A.;Markwald, Roger R.;Visconti, Richard P.
器官打印或计算机辅助机器人逐层添加生物制造厚的三维活组织结构采用自组装组织球体是一个快速发展的替代传统的固体支架为基础的方法在组织工程中。然而，缺乏在生物打印后立即加速组织成熟的有效方法是这项新兴器官打印技术进一步发展的主要技术要求和潜在障碍。确定加速组织成熟的因素和条件的最佳组合（“致成熟”因素）是一项必要和必要的努力。筛选成熟因子将最有效地完成使用高通量定量体外组织成熟试验。我们最近回顾了通过融合具有可测量材料特性的生物打印组织球体来形成固体无支架组织结构。我们假设这些组织球体的融合动力学将提供组织成熟水平的有效体外测定。在此，我们报告了两种简单的基于组织球体融合的体外高通量组织成熟筛选方法的实验测试结果：i)组织球体包膜法；ii)组织球体融合动力学试验。
Organ printing or computer-aided robotic layer by-layer additive biofabrication of thick three-dimensional living tissue constructs employing self-assembling tissue spheroids is a rapidly evolving alternative to classic solid scaffold-based approaches in tissue engineering. However, the absence of effective methods of accelerated tissue maturation immediately after bioprinting is the main technological imperative and potential impediment for further progress in the development of this emerging organ printing technology. Identification of the optimal combination of factors and conditions that accelerate tissue maturation (“maturogenic” factors) is an essential and necessary endeavor. Screening of maturogenic factors would be most efficiently accomplished using high throughput quantitative in vitro tissue maturation assays. We have recently reviewed the formation of solid scaffold-free tissue constructs through fusion of bioprinted tissue spheroids that have measurable material properties. We hypothesize that the fusion kinetics of these tissue spheroids will provide an efficacious in vitro assay of the level of tissue maturation. Herein, we report the results of experimental testing of two simple quantitative tissue spheroid fusion-based in vitro high throughput screening assays of tissue maturation: i) a tissue spheroid envelopment assay; and ii) a tissue spheroid fusion kinetics assay.
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影响因子：
6
作者：
Jian, B;Xu, J;Levy, RJ
通讯作者：
Levy, RJ
DOI：
10.1073/pnas.0400164101
发表时间：
2004-03-02
影响因子：
11.1
作者：
Jakab, K;Neagu, A;Forgacs, G
通讯作者：
Forgacs, G
影响因子：
20.1
作者：
Oka, Toru;Xu, Jian;Molkentin, Jeffery D.
通讯作者：
Molkentin, Jeffery D.
影响因子：
4
作者：
Robinson, EE;Zazzali, KM;Foty, RA
通讯作者：
Foty, RA
影响因子：
2.1
作者：
Marga, Francoise;Neagu, Adrian;Forgacs, Gabor
通讯作者：
Forgacs, Gabor